We have demonstrated the presence of a 210K (K = 10(3) Mr) microtubule-associated protein (MAP) in blood platelets and have studied its relationship to tubulin and to the cytoskeleton, using a well-characterized polyclonal antibody for the analysis. When platelet lysates were enriched for tubulin by an assembly cycle at 37 degrees C, the 210K MAP was also enriched, as detected by Western blotti...

Platelet calpain has many platelet substrates, including external membrane proteins. We thus investigated whether platelet calpain II was associated with platelet membranes in unstimulated and thrombin-activated platelets. A monospecific, goat polyclonal antibody was reared to purified platelet calpain II. Sixteen whole platelet lysates were found to contain 4.5 +/- 0.7 micrograms calpain antig...

Immune thrombocytopenia and pulmonary tuberculosis presented concurrently in a 20 year old Thai man as a bleeding diathesis. Intravenous immunoglobulin rapidly corrected the thrombocytopenia. Immunofluorescence and immunoblot studies with platelets and mycobacteria showed the presence of platelet surface membrane IgG.

Many methods have been described to identify platelet antibody, but they are either not very sensitive or too complex for general use. Therefore, we have developed an enzyme immunoassay for the detection of platelet antibodies in serum. The method involves incubating platelets with serum antibody; any attached antibody is shown by the addition of an enzyme (alkaline phosphatase) labeled anti-hu...