Background The utilization of immature oocytes derived from stimulated cycles could be of great importance, specially for urgent fertility preservation cases. The aim of the current study was to determine whether in-vitro maturation is more successful before or after the vitrification of these oocytes. MaterialsAndMethods The study was performed in a private IVF center. 318 germinal vesicle (GV...

in vitro maturation (ivm) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. this review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. the review is based on literature reports and the author’s experience. in ivm cycles,...

Background: 15% of oocytes collected in ART cycles are immature. These oocytes may be matured following IVM program. It is possible to cryopreserve the immature oocytes for further use in ART after application of IVM. The aim was to determine the maturation rate, morphology and viability of human immature oocytes after fresh IVM and vitrified-IVM program. Materials and Methods: 63 women who und...

The domestic cat experiences circannual variations in ovarian activity and intrafollicular oocyte quality. One result is poor nuclear and cytoplasmic maturation during in vitro maturation (IVM) conducted during the annual non-breeding season (July through November). In an attempt to overcome this seasonal phenomenon immature oocytes were collected from July through November and cultured in a co...

objective: crocin is an active ingredient of saffron (crocus sativus l.) and its antioxidant properties have been previously investigated. this carotenoid scavenges free radicals and stimulates glutathione (gsh) synthesis; consequently, it may protect cells against oxidative stress. the aim of this research is to protect oocytes from oxidative stress by the addition of a natural source antioxid...

BACKGROUND In assisted reproductive techniques, 85% retrieved oocytes are mature, and the rest are immature. These immature oocytes may be matured in vitro, and used in subsequent in vitro fertilization program. The purpose of this study was to determine the maturation capacity and morphology of human immature oocytes in both fresh and vitrified-thawed, in vitro matured oocytes with regard to t...

Background: The meiotic spindle plays an important role in the oocyte during chromosome alignment and separation at meiosis. The zona pellucid (ZP) is a dynamic matrix composed of filaments with the properties that might reflect the history of oocyte cytoplasmic maturation. Since, spindle and ZP in living oocytes are highly birefringent, their structures can be viewed noninvasively by using a P...

In Vitro Maturation (IVM) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. This review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. The review is based on literature reports and the author’s experience. In IVM cycles,...

  Background & Aim: Considering that antioxidants are known as effective free radicals scavenger, it may be able to improve the in vitro oocyte maturation and the fetal quality. This study was designed to determine the effect of Gundelia tournefortii leaves (GTE) hydro-alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: Germinal vasicle (GV) were...

objective: retinoids are recognized as important regulators of cell differention and tissue function. previous studies, performed both in vivo and in vitro, indicate that retinoids influence several reproductive events. in this study, we investigated the effect of all-trans retinoic acid (t-ra) on maturation and fertilization rate of immature oocytes (germinal vesicle). materials and methods: g...