OBJECTIVES To identify β-lactamase genes in gut commensal Bacteroides species and to assess the impact of these enzymes, when carried by outer membrane vesicles (OMVs), in protecting enteric pathogens and commensals. METHODS A deletion mutant of the putative class A β-lactamase gene (locus tag BT_4507) found in the genome of the human commensal Bacteroides thetaiotaomicron was constructed and...

Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603, formerly known as K. pneumoniae K6, is known for producing extended-spectrum β-lactamase (ESBL) enzymes that can hydrolyze oxyimino-β-lactams, resulting in resistance to these drugs. We herein report the complete genome of strain ATCC 700603 and show that the ESBL genes are plasmid-encoded.

The AmpC β-lactamases are clinically important because they confer resistance to the narrow-, expandedand the broad-spectrum cephalosporins, the β-lactam-β-lactamase inhibitor combinations and aztreonam. The clinical microbiology laboratories should be able to detect the bacterial strains which produce AmpC, since these strains may appear to be susceptible to a particular β-lactam antibiotic in...

OBJECTIVES The aim of this study was to detect the presence of IgG against Moraxella catarrhalis β-lactamase in healthy adults, and to determine whether outer membrane vesicles (OMVs) could protect the enzyme from inhibition by anti-β-lactamase IgG. METHODS Transmission electron microscopy was used to detect the presence of β-lactamase in OMVs. Sera were examined by ELISA for specific IgG dir...

AmpC β-lactamases are enzymes that resistant to β-lactams, such as penicillin and cephalosporin, but not cefoxitin cefotetan. This study was conducted characterize in Enterobacteriaceae. included 200 cephalosporin-resistant Gram-negative isolates recovered from different samples between January 2015 December 2016. The were subjected phenotypic tests, those tested positive further analyzed by PC...

conclusions biofilm formation (both strong and weak) strongly correlated with psla gene carriage. biofilm formation also correlated with mbl and ampc β-lactamase production. more importantly, multiple-β-lactamase phenotype was associated with formation of strong biofilms. results biofilm formation was observed in 43.5% of the isolates, of which 66.7% produced strong and 33.3% formed weak biofil...

Metallo-β-lactamases (MβL) catalyzing the hydrolytic cleavage of the four-membered β-lactam ring in broad spectrum of antibiotics and therefore inactivating the drug; However, the mechanism of these enzymes is still not well understood. Electronic structure and electronic energy of metallo-β-lactamase active center, two inhibitors of this enzyme including penicillin and cephalexin, and differen...

Two major hospitals in Kano, North West Nigeria have recorded increasing resistance of clinical pathogens to broad spectrum β lactams, mediated by extended spectrum β-lactamase (ESβL) and non ESBLs. A study was therefore undertaken to determine the occurrence and prevalence of plasmid and chromosomal mediated AmpC βL and carbapenemase in addition to already known ESBL due to increasing resistan...