We studied actin polymerization in the HL-60 human promyelocytic leukemia cell line during induced myeloid maturation and its relationship to the rate of locomotion (ROL). The percent G-actin (of total actin) was measured by DNAase I inhibition, F-actin was determined by fluorescence-activated cell sorter (FACS) analysis of nitrobenzoxadiazol (NBD)-phallacidin-stained cells, and ROL was measure...

Please cite this article in press as: M. Hammoud performance, Informat. Fusion (2013), http://dx One of the key challenges for research in wireless sensor networks is the development of routing protocols that provide application-specific service guarantees. This paper presents a new cluster-based Route Optimisation and Load-balancing protocol, called ROL, that uses various Quality of Service (Q...

Daar is alreeds oor die belangrikheid van terapeutiese gemeenskappe in die praktiese toepassing van psigiatrie geskryf. Die doel van hierdie artikel is om te illustreer in watter mate die rol van die psigiatriese verpleegkundige as terapeut in ’n terapeutiese gemeenskap ontwikkel het. Deur die inagneming van aspekte soos byvoorbeeld terapeutiese en dinamiese interaksie, terapeutiese inmenging o...

The methanol-regulated alcohol oxidase promoter (PAOX1) of Pichia pastoris is one of the strongest promoters for heterologous gene expression in this methylotrophic yeast. Although increasing gene dosage is one of the most common strategies to increase recombinant protein productivities, the increase of gene dosage of Rhizopus oryzae lipase (ROL) in P. pastoris has been previously shown to redu...

BACKGROUND The analysis of transcriptional levels of the genes involved in protein synthesis and secretion is a key factor to understand the host organism's responses to recombinant protein production, as well as their interaction with the cultivation conditions. Novel techniques such as the sandwich hybridization allow monitoring quantitatively the dynamic changes of specific RNAs. In this stu...

De rol van het motorsysteem tijdens taalbegrip Thesis To obtain the degree of Doctor from the Erasmus University Rotterdeam by command of the rector magnificus

Hereditary ataxic mice, tottering (tg) and rolling Nagoya (tg(rol)), carry mutations in the P/Q-type Ca(2+) channel alpha(1A) subunit gene. The positions of the mutations and the neurological phenotypes are known, but the mechanisms of how the mutations cause the symptoms and how the different mutations lead to various onset and severity have remained unsolved. Here we compared fundamental prop...

Phosphodiesterases (PDEs) are critical regulators of cyclic nucleotides in the heart. In ventricular myocytes, the L-type Ca(2+) current (I(Ca,L)) is a major target of regulation by PDEs, particularly members of the PDE2, PDE3 and PDE4 families. Conversely, much less is known about the roles of PDE2, PDE3 and PDE4 in the regulation of action potential (AP) properties and I(Ca,L) in the sinoatri...