نتایج جستجو برای: 16s rdna pcr
تعداد نتایج: 199975 فیلتر نتایج به سال:
All currently available DNA sequencing protocols rest fundamentally upon the homogeneity of the template. In this paper we describe the parallel DNA sequencing of various templates in one sample by a combination of the Sanger method and MALDI-TOF mass spectrometric analysis of the products. PCR-amplified hypervariable 16S rDNA fragments of the bacterium Escherichia coli DF1020 and cDNA of the 6...
AIM To measure the bacterial genome in ocular fluids and to analyse the clinical relevance of infectious endophthalmitis. METHODS Nineteen ocular fluid samples (eight aqueous humour and 11 vitreous fluid samples) were collected from 19 patients with suspected bacterial endophthalmitis. Fifty ocular samples from uveitis patients were also collected along with 40 samples from patients without o...
The identification of Mycobacteria in the species level has great medical importance. Biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. This research aimed to the comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria in patients suspected to tuberculosis in Golestan province which is the ...
BACKGROUND & OBJECTIVES The diagnosis of bacterial meningitis remains a challenge to the clinician because of its rapid lethal course lacking the consistency to particular clinical signs and symptoms. Moreover, in many clinical settings use of rampant and short course antibiotic therapy prior to lumbar puncture reduces the chance of isolation of bacteria in CSF culture making the diagnosis diff...
در این مطالعه، بر اساس منطقه 16s rdna باکتری شکمبه ای هضم کننده سلولز، بوتیریویبریو فیبری سالونس، یک روش واکنش زنجیره ای پلیمراز رقابتی (cpcr) بهینه سازی شد. آغازگرهای اختصاصی این باکتری برای تکثیر یک قطعه 213 جفت نوکلئوتیدی از ناحیه 16s rdna (dna هدف) از منابع انتخاب گردید. برای تولید قطعه رقابتگر همولوگ با قطعه هدف، دو آغازگر داخلی، که هر کدام حامل حدود 30 نوکلئوتید در انتهای ?5 خود بودند (به...
AIMS A new diagnostic strategy to improve the detection of pathogens in heart valves (HVs) from patients with infective endocarditis (IE) was evaluated. METHODS AND RESULTS Three hundred and fifty seven HVs surgically removed from 326 patients with proven IE or suspicious intra-operative findings, examined by 16S rDNA polymerase chain reaction (PCR) and culture were retrospectively analysed a...
Arachis hypogaea. L is a legume of economic importance, which is nodulated by Bradyrhizobium, a slow-growing bacteria. However there is no well characterization of this rhizobia in many areas of China. In the present study, cross-inoculation experiments were performed in cowpea and soybean. The isolated bacteria strains were characterized physiologyically, biochemically and id...
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