Dietary supplements and functional foods are becoming increasingly popular complements to regular diets. A recurring ingredient is the essential cofactor vitamin B12 (B12). Microalgae are making their way into the dietary supplement and functional food market but do not produce B12, and their B12 content is very variable. In this study, the suitability of using the human B12-binding protein int...

The novel tetrameric structure of human beta-tryptase faces each active site into the central pore, thereby restricting access of most biologic protease inhibitors. The mechanism by which the anti-tryptase mAb B12 inhibits human beta-tryptase peptidase and proteolytic activities at neutral pH, but augments proteolytic activity at acidic pH, was examined. At neutral pH, B12-beta-tryptase complex...

abstract bee venom is a complex mixture of proteins, peptides and low molecular components: proteins (enzymes) :( phospholipase, a2phospholipase b, hyaluronidase α – glucosidase) - , peptides (melittin, apamine mcd peptide,adolapine ,procamine a and b, protease inhibitors)- low molecular components: biogenic amines:( histamine, dopamine and noradrenalin) - pheromones: complex ethers- sugars :( ...

The natural vitamin B12-derivatives are intriguing complexes of cobalt that entrap the metal within strikingly skewed and ring-contracted corrin ligand. Here, we describe synthesis Rh(III)-corrin acetylrhodibalamin (AcRhbl) from biotechnologically produced metal-free hydrogenobyric acid analyze effect replacement cobalt-center organometallic B12-derivative acetylcobalamin (AcCbl) with its group...

difference in low results: t(22) 0.08 (P 0.43). The introduction and enforcement of guidelines produced a dramatic reduction in the number of B12 samples processed, with testing reduced by 70% over the first year. Only 14.3% of the overall reduction was due to rejected samples, indicating that the majority of the reduction was due to compliance with the guidelines by laboratory users. The reduc...

A review of how vitamin B12 is used by the human body and the role it plays in DNA mutation is presented. Vitamin B12 is a compound needed for healthy function of the human body but produced downstream of the point of absorption in the digestive system, rendering it unusable. One may become B12 deficient in a variety of ways including lack of intake, disruption of B12 absorption due to disease,...

Every year, thousands of basic military trainees in each service of the U.S. Armed Forces experience acute respiratory disease. The majority of this disease burden results from infection with human adenoviruses. We designed single- and multiplex assays that detect and discriminate adenovirus types B3, E4, B7, B11, B14, and B21. A total of 116 oropharyngeal swab specimens obtained from patients ...

The present study explored the efficacy of Bacillus spp. and protease production for biocontrol of the root-knot nematode Meloidogyne javanica in African violet media. Among 100 bacterial isolates from various soils, the highest nematode mortality was observed for treatments with isolate GM-18, which was identified as Bacillus subtilis based on cultural and morphological characteristics and 16S...