Regional lactate distribution in brain was assessed quantitatively in coronal sections using a bioluminescent technique. This bioluminescence can be induced by covering freeze-dried and heat-inactivated brain sections with a frozen solution containing enzymes and coenzymes both for lactate-dependent NADH formation and NADH-dependent bioluminescence, which was recorded photographically. Quantifi...

The histochemical localization of enzymes associated with purine nucleoside metabolism indicates that glial cells might participate in the regulation of these compounds in the central nervous system. In the present study we examined the histochemical localization of purine nucleoside phosphorylase (PNPase) in sections from adult rat brain. Some sections were also sequentially stained immunocyto...

background: the objective of the present study was to survey the presence of sarcocystis in sheep's brain in north khorasan province. methods: in general, 80 samples of sheep's brain were collected from slaugh-tered sheep in slaughterhouses of north khorasan province. tissue digestion method was used for observing bradyzoites in tissues. histopathological pro-cessing tracing sarcocystis and ens...

BACKGROUND The numerical density of synapses and their ultrastructural features are best assessed with electron microscopy. Counting is done within counting frames placed on a pair of sections (disector technique). But this requires that the thin sections are taken from comparable brain regions and the disectors are placed in a uniform random fashion. Small brain areas like the polymorph layer ...

A survey of existing atlases of the cat's brain has revealed a lack of coronal sections on the levels of the prefrontal cortex. On the other hand, neurophysiological and behavioral studies of this region have increased greatly in recent years. As the extent of coronal sections through the prefrontal cortex was seen to differ markedly even at separations of 1 mm, a stereotaxic atlas was made on ...

The main goal of this study was to develop a better light microscopic procedure for quantitative study of the cellular co-localization of neuropeptides in adult human brain tissue. To reach this goal, we opted for a method (proved to be optimal on rat brain) in which sections were double immunolabeled with two different fluorophore-conjugated secondary antibodies and analyzed with a confocal la...

Aberrant glycosylation is associated with most of the diseases. Direct imaging and profiling of N-glycans on tissue sections can reveal tissue-specific and/or disease-associated N-glycans, which not only could serve as molecular signatures for diagnosis but also shed light on the functional roles of these biomolecules. Mass spectrometry imaging (MSI) is a powerful tool that has been used to cor...

We consider the problem of aligning histological sections for 3D reconstruction and analysis. The method we propose is based on a blockmatching strategy that allows us to compute local displacements between the sections. We then collect these local measures to estimate a rigid transformation. Our emphasis is on the necessity to use a robust approach for this estimation step. The process is inte...

Direct immunofluorescence staining for herpes simplex virus was applied to cryostat sections of 43 specimens removed at brain biopsy. Herpes antigen was found in 10 specimens and virus was isolated from them. Antigen was found in one specimen from which virus was not isolated. Two specimens from which virus was isolated gave equivocal fluorescence. Thirty specimens gave no fluorescence or live ...