The transport of peroxisomal membrane proteins (PMPs) requires the soluble PEX19 protein as chaperone and import receptor. Recognition of cargo PMPs by the C-terminal domain (CTD) of PEX19 is required for peroxisome biogenesis in vivo. Farnesylation at a C-terminal CaaX motif in PEX19 enhances the PMP interaction, but the underlying molecular mechanisms are unknown. Here, we report the NMR-deri...

Membrane recruitment of coatomer and formation of coat protein I (COPI)-coated vesicles is crucial to homeostasis in the early secretory pathway. The conformational dynamics of COPI during cargo capture and vesicle formation is incompletely understood. By scanning the length of δ-COP via functional complementation in yeast, we dissect the domains of the δ-COP subunit. We show that the μ-homolog...

This paper aims to develop a multi-objective model for scheduling cargo trains faced by the costs of tardiness and earliness, time limitations, queue priority and limited station lines. Based upon the Islamic Republic of Iran Railway Corporation (IRIRC) regulations, passenger trains enjoy priority over other trains for departure. Therefore, the timetable of cargo trains must be determined based...

The objective of the present study was to describe the ecological status of ichthyofauna in an industrial district (Pará river, Amazon estuary), through the use of different environmental descriptors. To evaluate the impacts of the industrial area and cargo terminal, three areas were considered: Zone 1 (maximum impact), Zone 2 (median impact) and Zone 3 (low impact). A total of 77 species were ...

Due to the rapid increase in cargoes and postal transport volumes smart transportation systems, an efficient automated multidimensional terminal with autonomous elevating transfer vehicles (ETVs) should be established, effective cooperative scheduling strategy for needs designed improving cargo handling efficiency. In this paper, as one of most artificial intelligence technologies, bee colony a...

PEX5 acts as a cycling receptor for import of PTS1 proteins into peroxisomes and as a co-receptor for PEX7, the PTS2 receptor, but the mechanism of cargo unloading has remained obscure. Using recombinant protein domains we show PEX5 binding to the PEX14N-terminal domain (PEX14N) has no effect on the affinity of PEX5 for a PTS1 containing peptide. PEX5 can form a complex containing both recombin...

The size of endocytic clathrin-coated vesicles (CCVs) is remarkably uniform, suggesting that it is optimized to achieve the appropriate levels of cargo and lipid internalization. The three most abundant proteins in mammalian endocytic CCVs are clathrin and the two cargo-selecting, clathrin adaptors, CALM and AP2. Here we demonstrate that depletion of CALM causes a substantial increase in the ra...

Secretory proteins traffic from the ER to the Golgi via COPII-coated transport vesicles. The five core COPII proteins (Sar1p, Sec23/24p, and Sec13/31p) act in concert to capture cargo proteins and sculpt the ER membrane into vesicles of defined geometry. The molecular details of how the coat proteins deform the lipid bilayer into vesicles are not known. Here we show that the small GTPase Sar1p ...