Ghar-e-Dagh (Nitraria schoberi) is a native perennial plant profoundly resistant to salinity and drought. This plant, having a well-developed deep rooting system can act as sand dunes stabilization agent and also, due to its special alkaloids may perform a very significant role in pharmaceutical industries. Considering the importance of genetic engineering as a novel method to generate...

background:   objective: this study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis   materials and methods: isolated testicular cells were cultured in dmem/f12. characteristics of the isolated cells and obtained es-like cell were immune-cytochemically confirmed by examining the presence of plzf, vimentin, oct4 and nanog prote...

The mammalian ovary is composed of ovarian follicles, each follicle consisting of a single oocyte surrounded by somatic granulosa cells, enclosed together within a basement membrane. A finite pool of follicles is laid down during embryonic development, when oocytes in meiotic arrest form a close association with flattened granulosa cells, forming primordial follicles. By or shortly after birth,...

Osteoblasts (OBs) and osteoclasts (OCs) are 2 major groups of bone cells. Their cell-to-cell interactions are important to ensure the continuity of the bone-remodeling process. Therefore, the present study was carried out to optimize an OB/OC co-culture system utilizing the human OB cell line hFOB 1.19 and OCs extracted from peripheral blood mononuclear cells (PBMNCs). It was a 2-step procedure...

Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...

introduction: transplantation of germ cells restores the male fertility. nevertheless, a lot of questions remain incompletely resolved. the aim of this study was to evaluate in vitro colonization efficiency of germ cells and sperm production capacity of spermatogonial cells before and after culture by sperm number assay in epididymis of recipient mice. materials and methods: we developed a sert...

introduction: it is now generally accepted that vero cells provide beneficial effects on improving embryo quality in many animal species. the aim of this research was to determine the effect of vero cell co-culture on the development of vitrified 8-cell mouse embryos. material and methods: eight- cell mouse embryos were provided from superovulated female nmri mice and divided into treatment cas...

background: sertoli cells play a pivotal role in creating microenvironments essential for spermatogonial stem cells (sscs) self-renewal and commitment to differentiation. maintenance of sscs and or induction of in vitro spermiogenesis may provide a therapeutic strategy to treat male infertility. objective: this study investigated the role of luekemia inhibitory factor (lif) on the propagation o...