This paper reports on the optimization of conditions for the overproduction and isolation of two recombinant copper metalloproteins, originally encoded on the chromosome of the dentrifying soil bacterium Alcaligenes xylosoxidans, in the heterologous host Escherichia coli. The trimeric enzyme nitrite reductase (NiR) contains both type-1 and type-2 Cu centres, whilst its putative redox partner, a...

In-cell NMR allows characterizing the folding state of a protein as well as posttranslational events at molecular level, in the cellular context. Here, the initial maturation steps of human copper, zinc superoxide dismutase 1 are characterized in the E. coli cytoplasm by in-cell NMR: from the apo protein, which is partially unfolded, to the zinc binding which causes its final quaternary structu...

OBJECTIVE To evaluate the effects of copper-phenanthroline (CuOP) on pentachlorophenol (PCP)-induced adaptation and cell death of Escherichia coli. METHODS Bacterial growth and adaptation to PCP were monitored spectrophotometrically at 600 nm. Inactivation of bacterial cells was determined from colony count on agar dishes. Cellular ATP content and accumulation of PCP were assessed by chemilum...

This study was carried out to synthesize 1D inorganic nanostructure using an electrochemical method without any template and additives. Copper foils were anodized in a KOH bath and were tested for their antibacterial performance. After anodizing, the obtained samples were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD) to determine the corresponding morphology an...

We characterized the copper resistance genes in strain XvP26 of Xanthomonas campestris pv. vesicatoria, which was originally isolated from a pepper plant in Taiwan. The copper resistance genes were localized to a 7,652-bp region which, based on pulsed-field gel electrophoresis and Southern hybridization, was determined to be located on the chromosome. These genes hybridized only weakly, as dete...

Carboxymethyl chitosan dialdehyde starch Schiff base (CMCDAS) and its metal complexes are synthesized by corn (St), sodium periodate, carboxymethyl chitosan, ions (copper, zinc, nickel, silver), characterized Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analyzer (TGA), energy dispersive (EDS), X-ray powder diffraction (XRD). The antibact...

UNLABELLED To survive environmental challenges, biological systems rely on proteins that were selected by evolution to function in particular cellular and conditional settings. With the advent of protein design and synthetic biology, it is now possible to construct novel proteins that are not biased by eons of selection in natural hosts. The availability of these sequences prompts us to ask whe...