mycoplasma contaminants can be considered important not only for their roles as pathogens but also they may indicate that insufficient care has been taken during vaccine manufacture or quality control. the purpose of this study was to test the poliomyelitis vaccines for mycoplasma by culture and polymerase chain reaction (pcr) methods. during 2008 to 2009, a total of 47 lots of oral poliomyelit...

Abstract Background Brucellosis is endemic in Iran, and is seen in all age groups, including children. Its diagnosis in childhood needs a high index of suspicion. The diagnostic methods currently in use need evaluation for analytical performance. Objective The present study tries to evaluate the hematological indices, serologic markers of inflammation and symptoms in patients suspected of...

background detection of fastidious enteropathogenic bacteria in fecal samples of patients with gastroenteritis is a challenge in clinical microbiological laboratories. objectives the aim of this study was to compare the detection limits of the pcr and culture methods for the diagnosis of campylobacter spp., yersinia spp., clostridium perfringens, and clostridium difficile in human stool samples...

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

Streptococcus agalactiae , S. dysgalactiae and S. uberis are Gram-positive bacterial pathogens that affect cows in dairy herds. These are considered as the major causes of economic losses of dairy producers without a control program. To reveal the role of these agents in inducing subclinical mastitis in dairy cattle in Ahvaz, 100 CMT-positive milk samples and 20 CMT-negative milk samples were c...

abstract: ornithobacterium rhinotracheale (ort) has been identified as one of the emerging respiratory bacterial pathogens in turkey and chicken flocks. diagnosis of ort infection has been faced some difficulties in isolation and identification of the bacterium based on the biochemical properties. a reliable identification method that can be used in routine laboratory investigations is of impor...