A wide range of methods are currently available for determining the dissociation constant between a protein and interacting small molecules. However, most of these require access to specialist equipment, and often require a degree of expertise to effectively establish reliable experiments and analyze data. Differential scanning fluorimetry (DSF) is being increasingly used as a robust method for...

BACKGROUND Oscillations of the arterial partial pressure of oxygen induced by varying shunt fractions occur during cyclic alveolar recruitment within the injured lung. Recently, these were proposed as a pathomechanism that may be relevant for remote organ injury following acute respiratory distress syndrome. This study examines the transmission of oxygen oscillations to the renal tissue and the...

Diaminofluoresceins are widely used probes for detection and intracellular localization of NO formation in cultured/isolated cells and intact tissues. The fluorinated derivative 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM) has gained increasing popularity in recent years because of its improved NO sensitivity, pH stability, and resistance to photobleaching compared to the first-gene...

BACKGROUND Despite a plethora of monitoring techniques reported in the literature, only a small number of studies directly address clinical relevant end points, such as the flap salvage rate and false-positive rate. METHOD We conducted a systematic review of current evidence regarding the postoperative monitoring of microvascular free-tissue transfer via extensive electronic and manual search...

A study was done to evaluate the ingestion dose due to uranium in drinking water. The area of study is the twin cities of Hyderabad and Secunderabad, India. The uranium concentration in water samples was analysed by laser-induced fluorimetry. The associated age-dependent radiation dose was estimated by taking the prescribed water intake values and dose conversion factors for different age group...

In Egypt, nineteen water springs with temperatures exceeding 35 degrees C, which can be considered thermal waters, were reported. The radiological aspects of thermal water in three northern regions of Egypt (Gulf of Suez, Cairo, and Bahariya Oasis) were investigated. The activity concentrations of natural radionuclides of 226Ra, 40K, total uranium and 210Pb (210Po) in water samples were determi...