We attempted to determine the number of sperm cells required for genotyping of one microsatellite marker. The crude genomic DNA extracted from about 760 or more sperm cells gave sufficient quantity of PCR product using a 20 microl-scale PCR. We also studied the effects of non-ionic detergents on extraction of crude sperm genomic DNA. PCR products amplified with the crude sperm genomic DNA extra...

An automated procedure for NOE assignment and three-dimensional structure refinement is presented. The input to the procedure consists of (1) an ensemble of preliminary protein NMR structures, (2) partial sequence-specific assignments for the protein and (3) the positions and volumes of unassigned NOESY cross peaks. Chemical shifts for unassigned side chain protons are predicted from the prelim...

In the last years, there was an exponential increase in the number of publicly available genomes. Once finished, most genome projects lack financial support to review annotations. A few of these gene annotations are based on a combination of bioinformatics evidence, however, in most cases, annotations are based solely on sequence similarity to a previously known gene, which was most probably an...

One of the most important QC tests of whole-cell pertussis vaccine (WCPV) is potency test. In this regard, mouse protection test (MPT) is the current potency method, which is associated with severe animal distress and large variability in results. The purpose of this study was to assess Pertussis Serological Potency Test (PSPT) as a serological alternative method to intracerebral challenge in M...

Memes are the new social media language and they increasingly exercise their potency of effecting intense discourses among younger audience. In this dimension, seen as interesting resources communication that can promote L2 learning. English Language Acquisition (ELA) has always been a challenge for vernacular-medium Indian students. The study took 177 student participants (with background) pur...

background: malocclusion, as a worldwide issue, can cause a lot of problems for patients. panoramic (pr) and lateral cephalometric (lcr) radiographs are both usually indicated for orthodontic treatments. the aim of this study was to analyze vertical and sagittal parameters of both jaws on prs and to compare them on lcrs. materials and methods: in this cross sectional study, 61 samples from all ...

let r be a right gf-closed ring with finite left and right gorenstein global dimension. we prove that if i is an ideal of r such that r/i is a semi-simple ring, then the gorensntein flat dimensnion of r/i as a right r-module and the gorensntein injective dimensnnion of r/i as a left r-module are identical. in particular, we show that for a simple module s over a commutative gorensntein ring r, ...