By means of new or modified procedures troponin-tropomyosin complex was fractionated into three components plus tropomyosin, and then reconstituted in the absence of urea. Tropomyosin was separated from troponin by hydroxyapatite column chromatography, a method giving sharper separation than the previously used technique of isoelectric precipitation. The chromatography also separated the tropom...

BACKGROUND Shrimp is a frequent cause of food allergy. Tropomyosin is the major allergen in shrimp, and it shares homology to tropomyosins from other crustaceans, dust mites, cockroach, and parasites. OBJECTIVE The aim of this study was to determine the value of detection of IgE to shrimp tropomyosin in the diagnosis of shrimp allergy. METHODS We have studied 35 patients with asthma, rhinit...

Skeletal muscle beta-tropomyosin, smooth muscle alpha-tropomyosin, and a low molecular weight fibroblast tropomyosin are generated by alternatively splicing RNA transcripts of the chicken tropomyosin 1 (TM 1) gene (Forry-Schaudies, S., Maihle, N. J., and Hughes, S. H. (1990) J. Mol. Biol. 211; 321-330). Two novel tropomyosin cDNAs that derive from mRNAs of the TM 1 gene have been isolated from ...

The function of tropomyosinl in striated muscle remains undefined, although it has been su,, qvested that this protein might act to retard the interaction between actin and myosir? (2). The present investigation of the effects of tropomyosin on the behavior of actomyosin stems from a report that the properties of reconstituted actomyosin, prepared from purified actin and myosin, varied with dif...

Tropomyosin has been reported to be responsible for the cross-reactivity between shrimp and dust mites and the measurement of tropomyosin specific IgE was found superior to shrimp extract for the predicting of shrimp allergic reaction. A total of 55 dust mite allergic patients were recruited to analyse the cross-reactivity between shrimp and mites . Tropomyosin specific IgE for shrimps (rPen a1...

The intestinal epithelial cell brush border exhibits distinct localizations of the actin-binding protein components of its cytoskeleton. The protein interactions that dictate this subcellular organization are as yet unknown. We report here that tropomyosin, which is found in the rootlet but not in the microvillus core, can bind to and saturate the actin of isolated cores, and can cause the diss...

(1) Background: Tropomyosin is a major cause of shellfish allergy and anaphylaxis triggered by food. It acts as pan-allergen, inducing cross-reactivity in insects, dust mites, crustaceans, mollusks. Our study investigates children with asthma or atopic diseases after consuming tropomyosin-containing (2) Methods: We analyzed the molecular sensitization profiles pediatric patients at University C...

Myosin-II (Myo2p) and tropomyosin are essential for contractile ring formation and cytokinesis in fission yeast. Here we used a combination of in vivo and in vitro approaches to understand how these proteins function at contractile rings. We find that ring assembly is delayed in Myo2p motor and tropomyosin mutants, but occurs prematurely in cells engineered to express two copies of myo2. Thus, ...

The binding of 125I-labeled muscle tropomyosin to Acanthamoeba and muscle actin was studied by ultracentrifugation and by the effect of tropomyosin on the actin-activated muscle heavy meromyosin ATPase activity. Binding of muscle tropomyosin to Acanthamoeba actin was much weaker than its binding to muscle actin. For example, at 5 mM MgCl2, 2 mM ATP, and 5 micronM actin, tropomyosin bound strong...