Transport of amino acids into 3T3 and Py3T3 (polyoma virus-transformed 3T3 cells) was measured at preand postconfluent stages of their growth on glass coverslips. The 3T3 line is from mouse embryo and selected because of its strong “contact inhibition”. These cells, respectively, are and are not sensitive to density-dependent inhibition of growth, a phenomenon characterized by inhibition of cel...

A BALB/c 3T3 preadipose cell line defective in Na+K+Cl- cotransport (3T3-E12a cells) has been used to study the relationship between phorbol ester-induced rapid changes in cation fluxes and changes in expression of a gene known to be modulated by this agent. In contrast to its effect on parental 3T3 cells, 12-O-tetradecanoylphorbol-13-acetate (TPA) did not inhibit either furosemide-sensitive 86...

This paper reports that the angular distribution of directional change of 3T3 cells which followed guiding lines on a substrate and left the guidance at various angles shows a peculiar preference for angles between 30 degrees and 60 degrees. Regarding the phenomenon of cellular guidance itself, 3T3 cells faced with a choice between guiding lines toward different directions seem to explore vario...

La estructura de la ftalimida es considerada un bloque construcción para el desarrollo nuevos agentes anticancerígenos. En este trabajo, se evaluó actividad antiproliferativa cuarenta y tres derivados contra las líneas celulares cancerígenas cérvix (HeLa), hígado (HepG2), mama (4T1), línea celular normal fibroblastos murinos (3T3). Por último, realizó análisis acoplamiento molecular los en siti...

Background: Numerous molecules in Papaveraceae family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few cases. Objective: Numerous molecules in this family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a fe...

Hematopoiesis is regulated by two sets of signals, those generated by cytokines and those generated when precursor cells interact with bone marrow (BM) stroma. The intimate contact between precursors and stroma appears to be mediated by multiple, different receptor-ligand binding events. To identify receptor-ligand pairs mediating the adhesion of hematopoietic precursor cells to stroma, an in v...

Mammary epithelium differentiates in a stromal milieu of adipocytes and fibroblasts. To investigate cell-cell interactions that may influence mammary epithelial cell differentiation, we developed a co-culture system of murine mammary epithelium and adipocytes and other fibroblasts. Insofar as caseins are specific molecular markers of mammary epithelial differentiation, rat anti-mouse casein mon...

For 3T3, PY-3T3 and SV40-3T3 cells, the contents of total lipid, cholesterol and phospholipid were determined. Weight of total lipid per cell correlates closely with cellular volume as determined microscopically. This correlation is not observed for cholesterol of phospholipid content per cell. Cholesterol content per cm2 cellular surface area is found about fifty percent higher in the transfor...