Isoflavonoid contents of different plant parts and callus tissues of the Indian Kudzu, Pueraria tuberosa (Roxb.ex.Willd.) DC are presented. The initial cultures were slow growing, associated with browning of the tissues. The production of four isoflavonoids (puerarin, genistin, genistein and daidzein) in the callus cultures of P. tuberosa was studied by manipulating the plant growth regulators ...

Callus induction and withanolides production through cell suspension culture of Withania coagulans (Stocks) Dunal

Gymnema sylvestre (R.Br.) is an important diabetic medicinal plant which yields pharmaceutically active compounds called gymnemic acid (GA). The present study describes callus induction and the subsequent batch culture optimization and GA quantification determined by linearity, precision, accuracy, and recovery. Best callus induction of GA was noticed in MS medium combined with 2,4-D (1.5 mg/L)...

Ten commercial sugarcane varieties, N12, N14, N19, N24, N25, N26, N27, 75E247, 84E1334 and NCo376 were tested for suitability for transformation in terms of their embryogenic callus production and transient expression levels. Based on an increase in callus mass over time, varieties N12, N19, N27, NCo376 and 84E1334 outperformed other varieties on Murashige and Skoog medium containing 2,4-dichlo...

BACKGROUND AND AIMS Understanding the fate and dynamics of cells during callus formation is essential to understanding totipotency and the mechanisms of somatic embryogenesis. Here, the fate of leaf explant cells during the development of embryogenic callus was investigated in the model legume Medicago truncatula. METHODS Callus development was examined from cultured leaf explants of the high...

Background: Tissue culture is a new approach for production of secondary metabolites with many advantages over conventional methods. Elicitors such as nano particles are molecules that can stimulate the production of secondary metabolites. Objective: This study was conducted to evaluate the effects of some nano particles on mucilage production in Linum usitatissimum under tissue culture condit...

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

The present study is based on the in vitro production of callus, its long period maintenance and antibacterial potentiality. Callus induction and formation occurred by full strength MS media in combination with various concentration (0.5mg/l, 1.0mg/l, 1.5mg/l, 2.0mg/l) of 2, 4-D effective in combination with constant concentration of BA (2.5mg/l) and NAA (0.5mg/ml). The leaf calli were sub cult...

The low yield and high market price of the pharmaceutically important alkaloids of Catharanthus roseus (L.) G. Don viz. vincristine, vinblastine and ajmalicine have created interest in improved alternative routes for their production such as using cell and tissue culture. The callus developed on Murashige and Skoog (MS) media supplemented with different concentrations of auxins and cytokinins w...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...