Background: It was previously shown that the activity of a serine protease from a moderately halotolerant Bacillus aquimaris VITP4 strain is active in a wide range of pH and temperatures and could be modulated by the presence of the divalent metal ions.  Objectives: In the present study, a quantitative analysis was done in order to explore the parameters that are contributing to the protease a...

The main purpose of this project is investigation of the organophosphorus hydrolase (OPH) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the OPH enzyme stability in formulated creams. OPH enzyme was extracted and purified from strain flavobacterium. The w/o and o/w creams were prepared using different formulations. In order to achieve an emulsion with m...

Variations in phosphatidate phosphohydrolase (PAP) activity and triacylglycerol concentration were measured in pregnant and hormone-treated non-pregnant female rats. PAP activity in adipose tissue was elevated by 61% during pregnancy. The increase in the enzyme activity was paralleled with a rise in serum triacylglycerol concentration (44%). Estradiol injecting into non-pregnant rats increased ...

the inhibitory effects of several steroids on g6pd activity using intact erythrocytes are reported. incubation of whole blood with dehydroepiandrosterone (dhea) resulted in 42% and 12% inhibition in the enzyme activity in the presence and absence of oxygen, respectively. addition of epinephrine and/or aminophylline into the incubation medium caused further enzyme inhibition suggesting a possibl...

variations in phosphatidate phosphohydrolase (pap) activity and triacylglycerol concentration were measured in pregnant and hormone-treated non-pregnant female rats. pap activity in adipose tissue was elevated by 61% during pregnancy. the increase in the enzyme activity was paralleled with a rise in serum triacylglycerol concentration (44%). estradiol injecting into non-pregnant rats increased ...

alkaline protease enzyme produced from bacillus licheniformis ptcc1331 in optimum condition(temperature 37 ?c, 150 rpm, ph=10), is immobilized in different concentrations of sodium alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. maximum enzymatic activity of 46 was obtained in 2%w/v sodium alginate solution. investigat...

the snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. purification and partial characterization of a thrombin-like enzyme from the venom of the iranian snake, agkistrodon halys, was the aim of this study. purification was carried out by a combination of variety of chromatographic methods that included: gel fil...