gamma-aminobutyric acid is the main inhibitory amino acid neurotransmitter in several areas of the brain. measurement of gamma-aminobutyric acid in the brain will help elucidate its metabolic rote and diagnostic value. the liquid chromatography (lc) system consisted of a delivery pump. male rats (150-250g) were used in this study. the brain was rapidly removed from decapitated animals. trie chr...

Abbreviations: PTMs=post-translational modifi cations; PPIs=protein-protein interactions; SDS-PAGE= sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE=two-dimensional polyacrylamide gel electrophoresis; MS=mass spectrometry; ESI=electrospray ionization; MALDI=Matrix Assisted Laser Desorption Ionization; ESI-MS=electrospray ionization mass spectrometry; MALDI-MS=MALDI mass spectr...

separate alanine and arginine clearly. This problem was solved by the addition of 2 g/L potassium perchlorate as a chaotropic agent. The detection limits for all of the dabsyl derivatives, the data for regression analysis, and other statistics are listed in Table 2 in the Data Supplement that accompanies the online version of this Technical Brief at http://www. clinchem.org/content/vol50/issue2...

abstract mefenamic acid is a nonsteroidal anti-inflammatory drug (nsaid) that has analgesic,anti-infammatory and antipyretic actions. it is used to relieve mild to moderate pains. solid-phase extraction of mefenamic acid by a polymer grafted to silica gel is reported . poly allyl glycidyl ether/iminodiacetic acid-co-n, n-dimethylacrylamide was synthesized and grafted to silica gel and was used ...

a high performance liquid chromatography method was studied for determination of 18 ?-glycyrrhetinic acid in glycyrrhiza glabra l. (licorice) extract. the operating condition were c-18 reversed phase column (vp-ods, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/min (8-20min) and uv detection at 230 nm. the r...

A rapid, high-pressure liquid-solid chromatographic technique was developed for the separation, detection and analysis of ecdysteroids. The advantages in using this system for the identification of ecdysteroids in combination with NMR and mass spectrometry and its potential application in analyzing material from biological and synthetic,systems are presented. INTRODUCTION Although the molting h...