Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Horst Seidler’s curriculum vitae reminds the anthropologist of the early descriptions of human evolution, or, better, it is like the classic »hero myth«. The brave warrior sets out on his journey, seeks and faces and masters challenges along the way, and triumphs at the end. The heroic saga is a parable for Seidler’s scientific career that touches on several aspects of his personality: a deep e...

But Doctor, it's only Jimmie's right eye that has the cast. Why must you operate on the other one as well? How often has a puzzled parent confronted us with that question and, before answering, who among us has ever wondered what brave man first grasped his courage and advanced his scalpel at the " good eye "? That man died almost one hundred years ago; he lived in the then remote town of Carli...

parliament established jurisprudence of the islamic republic with due regard to the fundamental difference with conventional systems, is important. in conventional systems, the relationship between parliament and the people, legally determines representative role, responsibilities and his duties. but in the islamic republic of iran to the foundation fqhyash to find out the legal status and lega...

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

richard wright’s introductory essay to his collection of short stories uncle tom's children describes hidden forms of resistance against jim crowism and uncle tomism. the essay entitled the ethics of living jim crow: an autobiographical sketch relates wright’s daily confrontations with jim crowism with a particular emphasis on his evasive actions, and his covert activities, such as silence, pla...