Evidence of antibody isotype/subtype switching may provide prognostic value regarding the state of immune responses to therapeutic proteins, e.g. anti-factor VIII (FVIII) antibodies that develop in many hemophilia A patients, clinically termed "inhibitors". A sensitive, high- information-content surface plasmon resonance (SPR) assay has been developed to quantify IgG subtype distributions and t...

mumps infection is endemic in iran. our objective was to evaluate the presence of anti mumps antibodies ( igm & igg) in cerebrospinal fluid in mumps meningoencephalitic children. a prospective/cross-sectional study was performed in tehran, iran (2003 to 2004) and serum anti mumps antibodies (igm) were detected (quantitive; elisa) in meningoencephaltis patients. specific anti mumps antibodies (i...

Background & Objective: Toxoplasma gondii infection has public health importance and can lead to serious diseases in immunosuppressed patients, such as HIV cases. Appropriate control of T. gondii infection in HIV patients requires information about the prevalence of T. gondii antibodies and DNA in different population. In this study, we aimed to determine the prevalence of Toxo...

BACKGROUND Hirudin is a small protein with strong thrombin inhibition that may be antigenic. The generation and disappearance of anti-hirudin antibodies were investigated in patients with heparin-induced thrombocytopenia who were treated with recombinant hirudin (r-hirudin) for >/=5 days. METHODS AND RESULTS The IgA, IgE, IgG, and IgM isotypes of anti-hirudin antibodies were determined by ELI...

it has been also reported that that h. pylori infection may be responsible for some endocrine disorders, such as autoimmune thyroid diseases, diabetes mellitus and primary hyperparathyroidism. h. pylori which express cytotoxin-associated gene a (caga) may be more virulent than those that do not. the aim was to evaluate the seroprevalence of anti-h. pylori igg and anti-caga  antibodies in patien...

Uncoupling protein 1 (UCP1) expressed by the brown adipose tissue (BAT) in mitochondrial crista acts as a homeostatic thermogenerator of eutherians. The evaluation UCP1 expression BAT offers significant scientific insight, especially studies targeting limited areas such periarterial and pericardial regions small experimental mammals. However, negligible amount this would render general quantita...

Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for human IgG, IgA, or IgM in the IFA procedure. The effects of heat and mercaptoethan...

Background: Helicobacter pylori (H. pylori), is a common infection in pregnant women accompanied by variations in the levels of the IgM, IgA and IgG antibody isotypes. The variations of anti-H. pylori antibodies during and after pregnancy, and the extent of protection they provide to the mother and the fetus are not completely understood. Objectives: To investigate the changes of the anti-H. py...