background rapid detection of resistant strains of helicobacter pylori in human clinical samples is of major importance in clinical settings. inability of conventional clinical laboratory techniques in detection of these strains usually leads to failure of prescribed therapeutic regimens. objectives the aim of this study was designing a simple and rapid allele-specific pcr (as-pcr)-based method...

Single nucleotide polymorphisms (SNPs) of genes that affect cytokine production and function are known to influence the susceptibility and progression of immune-related conditions such as infection, autoimmune diseases, transplantation, and cancer. We established a multiplex genotyping method to analyze the SNPs of cytokine genes by combining the multiplex PCR and bead array platform. Thirteen ...

The loop-mediated isothermal amplification (LAMP) assay indicates a potential and valuable means for genetically modified organism (GMO) detection especially for its rapidity, simplicity, and low cost. We developed and evaluated the specificity and sensitivity of the LAMP method for rapid detection of the genetically modified (GM) maize T25. A set of six specific primers was successfully design...

the hla class ii genes of human major histocompatibility complex (mhc) exhibit an extensive degree of genetic polymorphism. this region subdivides into four sub-regions dp, do, dq, and dr. there are five different dr haplotypes that all contain a highly polymorphic allele called hla-drb1. in most recent studies, the association between hla-drb1 and a large number of diseases such as multiple sc...

With increasing knowledge about the causal role of genetic defects in clinical diseases the necessity is apparent to have procedures for rapid diagnosis of point mutations. We developed a PCR-based technique, whereby both normal and mutant alleles can be amplified in the same reaction tube, using different length allele-specific primers. Furthermore the allele-specific primers introduce additio...

Source and Description of Primers. Primers GHRE102′Fgc and GHRE102′R were designed based on the cDNA sequence of the bovine growth hormone receptor (GHR) gene (Hauser et al., 1990) and the genomic DNA sequence of the human GHR gene (Godowski et al., 1989). Primers GHRE10F and GHRE10R were designed based on our own sequence of the fragment amplified using the first pair of primers. Primer Sequen...

scid is a lethal genetic autosomal recessive disorder that has been observed in humans, dogs, mice, and horses. affected animals are incapable of generating specific antigens for immune responses needed to protect them from infectious diseases. the frequency of affected recessive allele varies in different regions so that the outcome of normal breeding with carriers of recessive alleles is diff...

We have developed a novel allele-specific primer elongation protocol using a DNA polymerase on oligonucleotide chips. Oligonucleotide primers carrying polymorphic sites at their free 3'end were covalently bound to glass slides. The generation of single-stranded targets of genomic DNA containing single nuclotide polymorphisms (SNPs) to be typed was achieved by an asymmetric PCR reaction or exonu...

Cancers arise from the accumulation of multiple mutations in genes regulating cellular growth and differentiation. Identification of such mutations in numerous genes represents a significant challenge in genetic analysis, particularly when the majority of DNA in a tumor sample is from wild-type stroma. To overcome these difficulties, we have developed a new type of DNA microchip that combines p...