New, quick, and inexpensive methods for genotyping novel caprine Fec gene polymorphisms through tetra-primer ARMS PCR were developed in the present investigation. Single nucleotide polymorphism (SNP) genotyping needs to be attempted to establish association between the identified mutations and traits of economic importance. In the current study, we have successfully genotyped three new SNPs ide...

The aim of this study was to develop primer pairs for Diplodia seriata identification, one of the most common fungal species associated with grapevine decline in Castilla y León (Spain). Genetic variability of selected isolates of D. seriata was estimated. A molecular marker was generated from a random amplified polymorphic DNA (RAPD) fragment. PCR products of around 1200 bp were obtained with ...

DNA fingerprinting has become an important tool for diversity assessment and varietal identification in plant breeding programs. Semi- random PCR primers targeting intron-exon splice junctions (ISJ) were used to evaluate the potential of these markers in identification and classification of rice genotypes. A total of 12 ISJ primers were used for screening fourteen Egyptian rice genotypes, inclu...

OBJECTIVE To evaluate the clinical performance of APC™II Victory Series™ (3M Unitek) brackets in direct orthodontic bonding with and without the use of primer. DESIGN A single-operator, two-centre prospective, non-inferiority randomized controlled clinical trial. SETTING The Orthodontic departments at the Leeds Dental Institute and St Luke's Hospital, Bradford, UK. ETHICAL APPROVAL Ethica...

Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...

We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.

Random amplified polymorphic DNA (RAPD) was used to study the genetic variations among strains from Fusarium sambucinum isolated from wheat in Upper Egypt. Using two different primer (V6 and M13), the used strains showed a high degree of similarities and a distinct RAPD patterns. The dendrogram that constructed based on M13 primers showed that, there is no correlation between isolation sources ...

CONTENTS SYNOPSIS INTRODUCTION WHAT ARE RAPDs Introduction Scoring RAPD Polymorphisms Sources of Polymorphism and Amplification Artifacts Population Genetic, Genealogical, and Phylogenetic Implications of Dominance and Cryptic Variation: A Preliminary Overview PROBLEMS WITH RAPD-PCR AND SOME REMEDIES Repeatability of Banding Patterns Within and Among Individuals Background Some Remedies Comigra...