The umbilical cord tissue has gained attention in recent years as a source of multipotent cells. Due to its widespread availability, the umbilical cord may be an excellent alternative source of cells for regenerative medicine. Anatomically, umbilical cord tissue is constituted of several different parts, and, accordingly, immunostaining of cord tissue sections revealed differential distribution...

background: human umbilical cord blood (hucb) is an acceptable and readily accessible source of stem cells. there is an ongoing interest in cord blood stem cell therapies; however, little is known about the possible unfavorable effects of laboratory modifications on the isolated hucb cells. the involvement of mirnas in several biological processes has been shown. the aim of this study was to ev...

background: in recent years we have successfully adopted an in vitro hepatogenic differentiation of mesenchymal stem cells (mscs). in this protocol the biologically active hepatocyte-like cells were differentiated from the stem cells isolated from either bone marrow or umbilical cord blood (ucb) samples. the aim of the present study was to compare the efficiency of the hepatogenic differentiati...

  Stem cells are naïve or master cells. This means they can transform into special 200 cell types as needed by body, and each of these cells has just one function. Stem cells are found in many parts of the human body, although some sources have richer concentrations than others. Some excellent sources of stem cells, such as bone marrow, peripheral blood, cord blood, other tissue stem cells and ...

............................................................................................................ 8 REVIEW OF THE LITERATURE .......................................................................... 9 1. UMBILICAL CORD BLOOD STEM CELLS .................................................. 9 1.1 Umbilical cord blood as a stem cell source .......................................... 9 1.2 ...

The paper presents current evidence on the properties of human umbilical cord-derived mesenchymal stem cells, including origin, proliferative potential, plasticity, stability of karyotype and phenotype, transcriptome, secretome, and immunomodulatory activity. A review of preclinical studies and clinical trials using this cell type is performed. Prospects for the use of mesenchymal stem cells, d...

There is currently great interest in the use of mesenchymal stem cells as a therapy for multiple sclerosis with potential to both ameliorate inflammatory processes as well as improve regeneration and repair. Although most clinical studies have used autologous bone marrow-derived mesenchymal stem cells, other sources such as allogeneic umbilical cord-derived cells may provide a more accessible a...

lipofectamine ltx is a cationic based transfection reagent that offers high levels of different transgenes expression in a huge number of cell types in vitro utilizing an easy and rapid protocol. the level of transgenes expression depend on some experimental variables containing cell density, lipofectamine and dna concentrations, lipofectamine -dna complexing time, and the presence or absence o...

Heart failure is one of the leading causes of death worldwide. End stage disease often requires heart transplantation, which is hampered by donor organ shortage. Tissue engineering represents a promising alternative approach for cardiac repair. For the generation of artificial heart muscle tissue several cell types, scaffold materials and bioreactor designs are under investigation. In this revi...