Salvia milthiorrhiza root (Danshen) is one of the oldest and most traditional drug of chinese origin, mainly used in the treatment of cardiovascular and cerebrovascular diseases. the tanshiniones (diterpenoids) and phenolic acids are the main biological active substances of S. miltiorrhiza. the aim of this study was to determine the optimal conditions for callus cultures and biosynthesis of the...

Successful efficient whole cauliflower plant regeneration via somatic embryogenesis from root derived callus tissue was achieved. The research confirmed for the first time the capability of mass production of cauliflower somatic embryos through the indirect pathway. The best callus induction and proliferation was on semi solid Murashige and Skoog (MS) medium supplemented with 2, 4-D at 0.15 mg ...

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

leaf and root explants from 10 to 20 day– old seedlings of plantago lanceolata, were cultured on ms, msh, nt and b5 basal media with various concentrations of different plant growth regulators. the best callus induction was obtained with 0.4 mgl-1 2,4-d in msh i with 400 callus index. callus growth was significantly stimulated (p=0.05)with 0.8 mgl-1 2,4-d and 0.1 mgl-1 kin in msh ii, with 7...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

An efficient procedure has been developed for callus induction and cell suspension cultures of C. saxicola for the first time. Explant selection was carried out among leaf, stem and root to select a suitable type of explants capable of higher callus formation. Leaf explants thus selected showed maximum response to callus induction (67.1%). Modified B5 medium supplemented with 0.5 mg l(-1) 2,4-D...

Since bone marrow has been shown to contain osteoprogenitor cells, an experiment was devised to test its effects when injected percutaneously into osteotomies sites in rabbit radii. In this experimental study, the osteogenicity and it's effect on early bone repair of bone marrow grafts were investigated. The purpose of this study was to determine whether bone marrow grafted percutaneously led t...

The effect of fungal elicitors and calcium-channel modulators on the anthocyanin production in callus cultures of Daucus carota was studied. It was found that the addition of fungal elicitors resulted in higher anthocyanin accumulation on the 3rd day of culture, but they resulted in lower levels of anthocyanin on the 21st day. Fungal elicitor treatment also affected growth of the cultures, as e...

in the present study, an efficient protocol has been developed for callus induction and production of ra in callus culture of satureja khuzistanica for the first time. in vitro callus induction was achieved from young shoot tip explants cultured on ms and b5 media supplemented with different concentrations of iba (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins bap and kin...

Various in vitro grown tissues (non-regenerative callus, regenerative callus and microshoot derived leaves) of Solanum nigrum L. were cultured under salinity stress (0-150 mM NaCl) for enhanced production of solasodine, a steroidal alkaloid and an alternative to diosgenin, which is used as a precursor for the commercial production of steroidal drugs. The role of plant growth regulators and vari...