Growth kinetics for Escherichia coli O157:H7 in perilla leaves were compared to those of pathogenic E. coli strains, including enteropathogenic (EPEC), enterotoxigenic (ETEC), enteroinvasive (EIEC) and other enterohemorrhagic (EHEC) at 13, 17, 24, 30 and 36 °C. Models for lag time (LT), specific growth rate (SGR) and maximum population density (MPD) as a function of temperature were developed. ...

AIM To determine the survival of Escherichia coli O157:H7 in dairy wastewater from on-site holding lagoons equipped with or without circulating aerators. METHODS AND RESULTS Survival was monitored in dairy lagoon microcosms equipped with or without scale-size circulators. Both laboratory strains of E. coli O157:H7 and an isolate of E. coli H7 from wastewater had poor survival rates and none p...

Title of Document: BIOFILM FORMATION BY ESCHERICHIA COLI O157:H7 Karen Suzanne Silagyi, M.S., 2007 Directed By: Associate Professor, Dr. Y. Martin Lo, Nutrition and Food Science Escherichia coli O157:H7 from cattle was evaluated for its ability to produce biofilm on food contact surfaces and quorum sensing signals in various raw meat, raw poultry, and produce broths. Generally, the strain was a...

conclusions the observed results indicated the necessity of good care in abattoir, butcheries and during food distribution, mainly ground beef. also, a zero tolerance policy could be useful to control e. coli o157 in meat products nationwide. results overall, 1.5% of ground beef samples were contaminated with the o157 e. coli strain meanwhile 1% of samples contained the o157:h7 strain and 0.5% ...

Enterohemorrhagic Escherichia coli O157 (EHEC O157) is a food-borne pathogen that has raised worldwide public health concern. The development of simple and rapid strain-typing methods is crucial for the rapid detection and surveillance of EHEC O157 outbreaks. In the present study, we developed a multiplex PCR-based strain-typing method for EHEC O157, which is based on the variability in genomic...

A nonpathogenic strain of Citrobacter sedlakii which expresses the Escherichia coli O157 antigen is described. The discovery of this strain emphasizes the necessity of additional biochemical and/or toxigenicity testing when isolates react with E. coli O157 latex reagents.

We compared fecal samples with samples collected with rectoanal mucosa swabs (RAMS) to determine the prevalence of Escherichia coli O157 in feedlot cattle (n = 747). Escherichia coli O157 was detected in 9.5% of samples collected with RAMS and 4.7% of samples tested by fecal culture. Pulsed-field gel electrophoresis analysis of isolates suggested that the strains colonizing the rectoanal juncti...

The behaviour of Escherichia coli O157:H7 was studied during the manufacture and storage of Iranian white cheese in brine. Cheese was manufactured using pasteurized cow milk and inoculated with E. coli O157:H7 with inoculum level of 103 cfu/ml. Four treatments were designed. Cheeses were made with or without starter culture and kept immersed in 6 or 8% salt brine during ripening and storage. Ch...

Three hundred rectal faecal samples and 213 raw milk samples obtained from the tanks and containers were examined using standard cultural methods. Escherichia coli O157:H7 was isolated from 11 (3.7%) of 300 faecal samples and 3 (1.4%) of 213 raw milk samples. It was determined that 8 (73%) of E. coli O157:H7 strains isolated from faecal samples originated from water buffaloes younger than 2 yea...