نتایج جستجو برای: fast pcr
تعداد نتایج: 401305 فیلتر نتایج به سال:
background and objective: bcg vaccination is used in many countries with a high prevalence of tb to prevent childhood tuberculosis meningitis and miliary disease. local and systemic sideeffects are associated with bcg vaccine. the most critical reaction is disseminated bcg infection which occurs in mostly immunodeficient patients. materials and methods: we performed 4 autopsies during 2001-2003...
BACKGROUND Tuberculosis (TB) remains as one of the most serious infectious diseases in the world. Pulmonary tuberculosis can occur with other pulmonary diseases caused by opportunistic organisms such as Nocardia spp. particularly in immunocompromised patients. Therefore, diagnosis of co-infection at the early stage of the disease could be lifesaving. OBJECTIVES The goal of this study was to d...
Pediatric pulmonary tuberculosis diagnosis is difficult because young children are unable to expectorate sputum samples. Testing stool for tuberculosis DNA from swallowed sputum may diagnose pulmonary tuberculosis. Hospitalized children with suspected tuberculosis had stool, nasopharyngeal, and gastric aspirates cultured that confirmed pulmonary tuberculosis in 16/236 patients. Twenty-eight sto...
مروری بر تکنیک های مولکولی در شناسایی سریع موتاسیون های منجر به مقاومت آزولی در قارچ های پاتوژن شایع
Invasive candidiasis and aspergillosis are amongst major medical concerns with high mortality among immunocompromised patients. Management of these infections is dependent on early and efficient antifungal therapy, as well as drug resistance monitoring. Decreased sensitivity of these pathogens to antifungal drugs during recent decades calls for rapid detection/identification of drug resistance ...
The early diagnosis of tuberculous meningitis (TBM) is very important. In this study, the efficiency of the polymerase chain reaction (PCR), one of the most reliable and sensitive DNA-based assays, was compared with conventional methods (acid-fast microscopy and culture) for the detection of M. tuberculosis in cerebrospinal fluid(CSF) specimens from patients suspected of TBM. Of the 29 CSF spec...
ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide coverage in most organisms. These short probes are highly specific and possess the high melting temperature (Tm) required for real-time PCR owing to the incorporation of locked nucleic acid (LNA). The ...
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