glutathione transferases are multifunctional proteins involved in several diverse intracellular events such as primary and secondary metabolisms, signaling and stress metabolism. these enzymes have been subdivided into eight classes in plants. the phi class, being plant specific, is the most represented. in the present study, based on the sequences available at genbank, different primers were d...

background: the present study sought to investigate molecular evidence for association between the presence of herpes simplex virus (hsv), cytomegalovirus (cmv), and epstein-barr virus (ebv) in crc and colorectal polyp by using the pcr method in iran. methods: in this analytical case-control study, we selected 15 patients with crc, 20 patients with colorectal polyp, and 35 patients without mali...

The rate of cervical cancer in Asians is deferent, based on the risk factors affecting and the rate of papiloma virus infection among females. Cervical cancer is one of the important cancers between Iranian women. In the present study the rate of cervical neoplasia was detected in Isfahan and some risk factors affecting the incidence of cervical cancer was analyzed. The human papiloma virus inf...

abstract genomic dna extraction with high quantity and quality is the basic necessities of molecular genetic studies. the aim of this study is the applicability of different dna extraction methods as the easiest and fastest methods for and their efficiency with different tissue qualities (foot, siphon, harpoon) from marine cone snail conus coronatus, mollusca with phenol-chloroform, ammonium ac...

Paraffin-embedded necropsy material from 6 patients with human cytomegalovirus encephalitis (HCMVE) corroborated by immunocytochemistry and 11 control cases were examined for the presence of human cytomegalovirus (HCMV) DNA by a nested polymerase chain reaction (nPCR). A characteristic 183 base pair (bp) fragment of the HCMV genome could readily be amplified in 4 cases of HCMVE. In 2 cases of H...

BACKGROUND Next-generation sequencing (NGS) technologies have changed our understanding of the variability of the human genome. However, the identification of genome structural variations based on NGS approaches with read lengths of 35-300 bases remains a challenge. Single-molecule optical mapping technologies allow the analysis of DNA molecules of up to 2 Mb and as such are suitable for the id...

Analysis of DNA and/or RNA is an important component of most epidemiologic studies. The methods used for their preparation vary depending on the number of samples in the study as well as the amount of tissue or cells available, the specific downstream assay, and the resources available. They range from classic phenol/chloroform extractions to robotic methods suitable for large-scale studies. Se...

Cowpea is one of the most consumed food in Africa. Recombinant DNA technology has been used to insert foreign genes into plants genome via cassettes there by creating a new generation with desired traits. Such possess improved seed quality, yield and resistance pests pathogens. The aim this study screen for transgene Cowpeas sold Nigerian markets using molecular analysis. Five Improved varietie...

The JC polyomavirus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease. The current diagnostic standard for PML is real-time PCR testing of extracted DNA for assessing the presence of JCV DNA in cerebrospinal fluid (CSF). This study was aimed at evaluating the feasibility of a real-time PCR assay without nucleic acid extraction for th...