A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.

hbme-l is an antimesothelial monoclonal antibody that recognizes an unknown antigen on microvilli of mesothelial cells. the antibody is only relatively specific for mesothelium and is used in the differential diagnosis of mesothelioma and adenocarcinoma within the context of an appropriate immuno-histochemical panel. hbme-l has also been reported to strongly and uniformly stain papillary and fo...

OBJECTIVE To determine the correlation of Gram staining with the culture of bronchoalveolar lavage in patients with clinical features of ventilator-associated pneumonia from two adult trauma and surgical intensive care units. METHODS We conducted a retrospective study of 252 samples of bronchoalveolar lavage from patients with clinical ventilator-associated pneumonia in trauma or surgical pos...

Among different staining methods used to demonstrate the nuclear abnormalities, Feulgen is one of the most reliable method. Feulgen staining is specific, sensitive method for evaluating the DNA damages.It has been shown that using non-DNA specific stains for monitoring the nuclear anomalies lead to false-positive or false-negative results. From self-experience, immersing the stained slides in h...

background : this study aimed to compare three staining methods including: calcofluor white, chromotrope and quick hot gram chromotrope used in diagnosis of intestinal microsporidial spores . methods : one hundred and seventy five stool specimens were collected from patients referred to laboratory of intestinal protozoology at the school of public health, tehran university of medical sciences d...

Methods An ongoing prospective study conducted from June 2012October 2013 in 90 HIV seropositive patients presenting with various complaints. CSF and lymphnode aspirates were subjected to preliminary microscopic examination, c (Giemsa staining, mucicarmine staining, negative staining and Gram’s staining). Latex agglutination test was done for CSF samples. Sampls inoculated on SDA at 37C and 25C...

By comparing in situ negative staining of mammalian heart muscle using La(NO3)3 with conventional positive staining by uranium and lead salts, we showed that 1) the membrane area of rat cardiac gap junctions (GJs) at the intercalated disks is threefold to fourfold greater than previously thought; 2) connexon arrays of cardiac GJ are subdivided into microdomains by connexon-free aisles; 3) profi...