Human luteal cells have been reported to express human leukocyte antigen-DR and lymphocyte functional antigen-3 on the cell surface, suggesting physiological interaction between luteal cells and T-lymphocytes through the menstrual cycle into early pregnancy. To elucidate the role of peripheral lymphocytes on corpus luteum differentiation, the effect of peripheral blood mononuclear cells (PBMC) ...

A comparative analysis was performed of the phenotype and function of peripheral blood leukocytes of two age-matched cohorts of industrial workers in chemical plants, one of which was exposed occupationally to high concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Median actual TCDD burdens were 116 ng/kg and 4 ng/kg, respectively. The phenotype analysis of peripheral blood mononucl...

The patterns of gene expression and the phenotypes of lymphocytes in peripheral blood mononuclear cells (PBMC) from children with diarrhea caused by rotavirus and healthy children were compared by using DNA microarray, quantitative PCR, and flow cytometry. We observed increased expression of a number of genes encoding proinflammatory cytokines and interferon or interferon-stimulated proteins an...

THIS panel was composed and optimized to investigate the differentiation stages of human cd T-cells in cryopreserved peripheral blood mononuclear cells (PBMC) from healthy individuals (Tables 1 and 2). As the majority of pan-cd T-cell antibodies available commercially proved to be inappropriate for detecting all cd T-cell populations in combination with other markers, this panel provides an ess...

BACKGROUND During the establishment of the maternal blood circulation around the implanting human embryo, maternal peripheral blood mononuclear cells (PBMC) directly contact trophoblasts. To determine the physiological significance of this interaction, the effects of PBMC obtained from pregnant women on the proliferative and invasive properties of a human choriocarcinoma cell line, BeWo cells, ...

Immunotherapy is effective in treating patients with a variety of cancers We developed and employed an immunotherapeutic protocol that combined intra-tumor injection autologous immature dendritic cells , standard care radiation to treat different advanced solid tumors. Pre post treatment peripheral blood mononuclear ( PBMC) were assayed for their capacity kill tumor cell lines had been establis...

Submit Manuscript | http://medcraveonline.com Abbreviations: PBMC: Peripheral Blood Mononuclear Cells; TME: Tumor Microenvironment; FFPE: Formalin-Fixed ParaffinEmbedded; OCT: Optimal Cutting Media; RNA: Ribonucleic Acid; DNA: Deoxy Ribonucleic Acid; TCR: T Cell Receptor; TIL: Tumor Infiltrating Lymphocytes; IHC: Immunohistochemistry; Cytof: For Cytometry by Time of Flight; Elispo: Enzyme Linke...

Current laboratory lymphokine-activated killer (LAK) cell activation procedures require culture of peripheral blood mononuclear cells (PBMC) in the presence of 1000-1500 units/ml of interleukin 2 (IL-2) for 3-7 days. However, we have observed that a brief exposure (15 min-1 h) of PBMC to a high concentration of IL-2 results in the maturation of LAK precursor cells to cytolytic effector cells ov...

Background The Cancer Immunotherapy Trials Network has initiated several clinical trials utilizing T cell growth factors such as IL-15 and IL-7. In support of these trials, we have developed and conducted precision testing of a 12-color flow cytometric assay to characterize the surface markers present on T cells in fresh whole blood and among cryopreserved peripheral blood mononuclear cells (PB...