Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which provides high sensitivity and resolution, has been used for the analysis of biopolymers. In past decade, MALDI-TOF MS utilized in clinical fields around world to identify microbial species. However, serotype or strain discrimination same bacterial species is difficult by MS. Different methods such...

Constant antigenic drift of circulating influenza viruses leads to inefficiency seasonal vaccines, thus requiring annual re-design these vaccines. Therefore, the development a universal vaccine is particular relevance. A promising line research in this area design immunogens consisting conserved protein fragments from different viral strains. The aim work was assess immunogenicity DNA vaccines ...

Abstract Background To explore the differences of multiple cytokines secretion based on QuantiFERON-TB Gold Plus(QFT-Plus)antigens stimulation, and to screen with potential differentiate tuberculosis infection status. Methods Twenty patients diagnosed microbiologically active tuberculosis(ATB)in Peking Union Medical College Hospitaland Beijing Chest Hospital from February April 2022 were enroll...

Most advanced vaccines against severe acute respiratory syndrome coronavirus (SARS-CoV)-2 are designed to induce antibodies spike (S) protein. Differently, we developed an original strategy CD8+ T cytotoxic lymphocyte (CTL) immunity based on in vivo engineering of extracellular vesicles (EVs). This is a new vaccination approach intramuscular injection DNA expression vectors coding for biologica...

conclusion the identification of immunodominant proteins adapted to the physiological and genetic conditions of the host is an appropriate diagnostic approach, which could be associated with improved sensitivity and specificity of serologic tests. results four immunodominant proteins (23, 28, 30, 41 kda) were recognized with strongyloidiasis sera in 0.1 diluted serum. none of the proteins react...

Background: The honey bee of the Apis mellifera species is main type cultivated in almost all countries world, including Indonesia. Honey has benefits various aspects, food, health, and beauty. Royal jelly a daily food menu as supplement to maintain increase health vitality. Purpose: Analyzing immunogenic toxinogenic proteins carpatica immunotherapy. Method: Conversion nucleotides into amino ac...

background: patients with ulcerative colitis (uc) carry autoantibodies such as perinuclear antineutrophil cytoplasmic antibodies (p-anca). objective: the aim of the present study was to evaluate the target antigens for p-anca in iranian patients with uc. methods: p-anca target antigens including elastase, lactoferrin, cathepsin g, myeloproxidase, lysozyme, and bactericidal permeability increasi...

the objective of this study was to determine the protein pattern and antigenic structure of bhv-1. ten viral isolates from cows with clinical signs of respiratory infections were selected for this study and confirmed by sn test. all samples were cultured on cell cultures and purified by ultracentrifugation. purified isolates were electerophorsed using sds-page method and immunoblotted. viral an...

Phage-derived affinity peptides have become widespread thanks to their easy selection via phage display. Interactions between a target protein and its specific peptide are similar those antibodies antigens. The strength of these non-covalent complexes may be described by the dissociation constant (Kd). In this paper, protein-specific exposed on pIII present in M13 bacteriophage virion with up f...