Hypocholesteremic and antioxidant effects of Withania somnifera (WS) Dunal (Solanaceae) were investigated in hypercholesteremic male albino rats. When the root powder of WS was added to the diet at 0.75 and 1.5 gm/rat/day, hypercholesteremic animals registered significant decreases in total lipids (-40.54%; -50.69%), cholesterol (-41.58%; -53.01%) and triglycerides (-31.25%; - 44.85%) in plasma...

an improved and facile preparation of 3,7-dinitro-1,3,5,7-tetraazabicyclo [3,3,1] nonane(dpt) has been developed starting from urea. in the procedure dpt was synthesized vian,n’-dinitrourea (dnu) as intermediate and various operating parameters such astemperature, ph and molar ratio of reagents have been optimized to give maximum yield ofthe desired product. the low cost method is concise, oper...

Simvastatin (SIM) (Figure 1) reduces levels of circulating atherogenic lipoproteins by competitive inhibition of the microsomal enzyme 3-hydroxy-3-methylglutaryl-co-enzyme A(HMG-CoA) reductase, which catalyzes the conversion of HMG-CoA to mevalonate, a critical intermediary in the biosynthesis pathway of cholesterol1. Also SIM inhibits oxidation of native and modified low-density and high-densi...

Procedures were developed for the determination of the activity of the microsomal enzyme 3-hydroxy-3-methylglutaryl CoA reductase (HMG CoA reductase, EC 1.1.1.34) in human liver. The enzyme assay could be carried out with as little as 20 mg of fresh liver tissue, thus making the method applicable to specimens obtained by percutaneous liver biopsy. Experiments were carried out to determine optim...

The Mycobacterium tuberculosis genome contains about 4000 genes, of which approximately a third code for proteins of unknown function or are classified as conserved hypothetical proteins. We have determined the three-dimensional structure of one of these, the rv0216 gene product, which has been shown to be essential for M. tuberculosis growth in vivo. The structure exhibits the greatest similar...

Methods were developed for determination of microsomal HMG-CoA reductase activity from freshly isolated human lymphocytes, monocytes, and granulocytes or cultured human lymphoid cells. Reductase activity in monocytes is approximately twice that in lymphocytes or granulocytes. The activity in cultured cells is approximately 34-fold greater than that in freshly isolated cells. Assay conditions we...