dna sequence determination is a tremendous human achievement. dna sequencing includes several methods and technologies in use for determining the order of the nucleotide bases (adenine, guanine, cytosine, and thymine) in a molecule of dna. knowledge of dna sequences has become indispensable for basic biological research, other research branches utilizing dna sequencing, and in numerous applied ...

We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease.

DNA is the ultimate molecule that encodes life through genes. Genes positioned along meter-long have to be expressed at right time and in amount via strict regulatory processes. Regulation of a gene starts with binding DNA-binding protein known as transcription factor (TF) target element double-stranded molecule. Often, TFs attach sequence-specific manner can motifs various lengths, yet some al...

Prior to the production of human gamma interferon using recombinant DNA technology, it had been producedmainly upon mitogenic induction of lymphocytes in very low amounts, which evidently hamperedits characterization and its medical applications. The recombinant gamma interferons produced in largerquantities in prokaryotic systems retain their biological activities, and can be...

serology and dna techniques are employed for human leukocyte antigen (hla) typing in different transplant centers. results may not always correlate well and may need retyping with different technique. all the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring hla typing for bone marrow transplant were enrolled in the study. serological hla typing was ...