BACKGROUND Recent studies have demonstrated that morphology of the first polar body (1(st)PB) is related to oocyte viability, which can be used as a prognostic tool to predict oocyte performance and pregnancy outcomes in an intracytoplasmic sperm injection (ICSI) program. According to some studies, there is a correlation between oocyte performance and 1(st)PB morphology, while others have not r...

BACKGROUND GJA1 and PTX3 were proposed as gene markers for oocyte and embryo developmental competence, while SERPINE2 was reported to be associated with pregnancy outcome. PRSS35, which is exclusively expressed in the ovary, may be correlated with oocyte competence. This study was conducted to evaluate the correlation of cumulus GJA1, PRSS35, PTX3, and SERPINE2 gene expression levels with oocyt...

BACKGROUND Embryo morphology has been proposed as an alternative marker of chro- mosomal status. The objective of this retrospective cohort study was to investigate the association between the chromosomal status on day 3 of embryo development and blas- tocyst morphology. MATERIALS AND METHODS A total of 596 embryos obtained from 106 cycles of intra- cytoplasmic sperm injection (ICSI) followed...

Organ and tissue formation are complex three-dimensional processes involving cell division, growth, migration, and rearrangement, all of which occur within physically constrained regions. However, analyzing such processes in three dimensions in vivo is challenging. Here, we focus on the process of cellularization in the anterior pole of the early Drosophila embryo to explore how cells compete f...

background: all-trans retinol is a biological antioxidantscavenging the ros in the cell culture. objectives: this studywas conducted to investigate the effect of all-trans retinol infertilization and culture medium on mouse embryo's developmentalcompetence. methods: this study was designed into twoexperiments. in the first experiment, in vitro mature oocytes wereco-cultured with sperm in fertil...

The current systematic review was aimed to assess the effectiveness of the zygote morphology evaluation in fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles. All available studies reporting on zygote morphology and clinical and/or biological outcomes were analyzed. Forty studies were included in the final analysis. Fourteen different zygote scoring systems we...

Many strategies have been proposed for the selection of viable embryos for transfer in human assisted reproduction. These have included morphological scoring criteria for 20, 28, 44 and 68 h after insemination. The embryo selection is based on morphology, degree of fragmentation and development to the 8-cell. All have shown some correlation with implantation. However, the overall success of the...

The development of an organism is accompanied by various cellular morphogenetic movements, changes in cellular as well as nuclear morphology and transcription programs. Recent evidence suggests that intra and inter-cellular connections mediated by various adhesion proteins contribute to defining nuclear morphology. In addition, three dimensional organization of the cell nucleus regulate the tra...

Prior to ICSI the cumulus-oophorous-complex of the oocyte is commonly remove with impure animal-derived hyaluronidase of low strength. The lower strength of the enzyme, the high concentration of impurities and the necessity of extensive time-dependent mechanical denudation damages of the oocytes, increases the risk of lysing the oocytes and the transfer of animal-derived pathogens. All this can...

Flowering plants, like placental mammals, have an extensive maternal contribution toward progeny development. Plants are distinguished from animals by a genetically active haploid phase of growth and development between meiosis and fertilization, called the gametophyte. Flowering plants are further distinguished by the process of double fertilization that produces sister progeny, the endosperm ...