background: this study was conducted to evaluate fibroblast co-culture and activin a on in vitro maturation and fertilization of mouse preantral follicles. methods: the ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-mem as based medium for 12 days. a total number of 456 follicles were cultured in four conditions: (i) base mediu...

Failure to select a viable embryo in a given fertility treatment cycle limits the success rates of current procedures. Fertility clinics rely on a morphological assessment of embryo appearance at a few distinct timepoints during development to pick a viable embryo for transfer. However, embryo development is a very dynamic process and critical stages between observations go unnoticed. This docu...

introduction: consumption of morphine, during pregnancy, in addition to inducing defects in the mother’s nervous system function, caused defects or delays in the formation and evolution of embryonic visual system. in the present study, changes in lens development were assessed in embryos exposed to morphine in utero. methods: female wistar rats (250-300 g) were mated with male rats and pregnanc...