A repetitive sequence-based PCR (rep-PCR) technique was developed to characterize the genotypic relatedness among Candida rugosa isolates. Two repetitive sequences, viz., Care-2 and Com29 from Candida albicans, were used to design primers Ca-21, Ca-22, and Com-21, respectively. When used alone or in combination, these primers generated discriminatory fingerprints by amplifying the adjacent vari...

to investigate the genetic diversity of 21 rapeseeds (brassica napus l.), 20 semi-random isj and ssr primers were used. primers produced a total number of 158 (81.5%) polymorphic bands (51 ssr and 107 isj bands). average numbers of polymorphic bands for isj, ssr and combined of them were 10.7, 5.1 and 7.9, respectively. pic values ranged from 0.0150 for specific primer o110d08 to 0.3420 for sem...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study the polymorphism of the cast gene ofkhalkhali goat in azerbaijan province in iran was investigated by polymerase chain reaction and restriction fragment length polymorphism technique (pcr-rflp). genomic dna was extracted from whole blood samples collected from 200 kh...

objective: utility of pcr-rflp and species-specific pcr as novel and fast methods for identification and discrimination of causative agents of relapsing fever, borrelia persica and b. microtii in infected blood were investigated. materials and methods: genomic dna of b.persica and b.microtii species were extracted from the highly infected blood samples. two fragments of glpq and 16srdna genes ...

PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-a...

wild grasses are the most important primary feedstuffs which are susceptible to contamination with toxigenic fungi belonging to aspergillus spp. in order to explore diversity of aspergillus species associated with the inflorescences of gramineous weeds, infected inflorescences were collected from wild grasses in western parts of iran. fifty-six aspergillus isolates were obtained from all diseas...

background and aims: tomato yellow leaf curl virus (tylcv) is one of the most destructive viruses of tomato that leads to reduced tomato yield up to 100% in tropical and subtropical regions. in this study, the complete sequence of tylcv isolate from hormozgan province, iran and its recombination evsent was determined. methods: tylcv infected tomato was collected from hormozgan province. total d...