Serological survey on the occurrence of alpha-herpesvirus and pestivirus infections in the population of European bison in the Bialowieza Primeval Forest was conducted. Sera from 40 randomly selected individuals were tested using two commercial IDEXX ELISA tests. The study design allowed excluding or confirming the infection with the probability of 95% and at the expected prevalence of 30%. No ...

BACKGROUND Infection due to bovine viral diarrhoea virus (BVDV) is endemic in most cattle-producing countries throughout the world. The key elements of a BVDV control programme are biosecurity, elimination of persistently infected animals and surveillance. Bovine viral diarrhoea (BVD) is a notifiable disease in Belgium and an official eradication programme started from January 2015, based on te...

The polymerase chain reaction was used to detect genomic sequences of the positive-stranded RNA of bovine viral diarrhea virus (BVDV), a member of the family Togaviridae. Using a set of 20-bp primers located within the conserved 3' region of the BVDV genome, we were able to consistently amplify a 205-bp target sequence from BVDV cDNA. BVDV RNAs from cell culture-propagated BVDV reference strain...

Commercial enzyme-linked immunosorbent assays (ELISAs) for detection of serum antibodies to bovine viral diarrhea virus (BVDV), parainfluenza-3 virus (PI3V), respiratory syncytial virus (RSV), and infectious bovine rhinotracheitis virus (IBRV) were standardized to give a quantitative result when testing was performed at a single optimum dilution. For each test, serum samples were titrated and t...

background: bovine viral diarrhea virus (bvdv) causes significant economic and health effects in cattle farms. because of the relative inefficiency of bvdv eradication programs, in recent years, numerous strategies such as anti-viral gene therapy has been used extensively to fight it. one of the ways to evaluate the quality of these anti-viral strategies is the study of their efficacy in the sp...

Bovine viral diarrhea viruses (BVDV) are members of the Pestivirus genus within the family Flaviviridae. Based on antigenic and nucleotide differences, BVDV are classified into two recognized species, BVDV-1 and BVDV-2. More recently, a new putative pestivirus species, tentatively called "HoBi-like", has been associated with bovine viral diarrhea. HoBi-like viruses were first identified in feta...

This dissertation summarises the findings of two separate studies relating to the epidemiology of bovine viral diarrheoa virus in New Zealand dairy herds. The objective of the first study was to estimate the distribution and causes of abortion in dairy herds across New Zealand during the 2001/2002 breeding season. A questionnaire survey was sent to all veterinary practices that were listed as m...

biv is a well-known bovine immunosup-pressive cause, but its pathogenesis has not been well characterized. it seems that it is possible that cofactors such as co- infection with other bovine viral pathogens may play a role in enhancing the pathogenesis of biv infection; bvdv also has immunosuppressive effects. objective: the aim of this study was determination of possible correlation between bi...