It had long been assumed that a bacterial cell was dead when it was no longer able to grow on routine culture media. We now know that this assumption is simplistic, and that there are many situations where a cell loses culturability but remains viable and potentially able to regrow. This mini-review defines what the "viable but nonculturable" (VBNC) state is, and illustrates the methods that ca...

Canlı fakat kültürü yapılamayan (VBNC) durum henüz moleküler mekanizması çözülememiş bir süreçtir. Bu çalışmada iki bileşikli fosforlama sistemlerinden birisi olan CusS-R’nin alkalin pH (8.5) ortamında VBNC forma geçişte rollerinin olup olmadığı araştırılmıştır. Çalışmada elde edilen sonuçlara göre hem cusS de cusR genlerinin nakavt edilmesi 7.2 etki göstermezken, E. coli‘nin alkali ortamda yaş...

Enterococcus faecalis is frequently recovered from refractory endodontic infections and has been implicated in endodontic treatment failures. This study compared real-time quantitative PCR (qPCR) assay to cultivation for E. faecalis detection and quantitation during endodontic treatment. A reverse-transcription PCR (RT-PCR) assay was also developed to detect the bacterium clinically in the viab...

Several antimicrobial urinary catheter materials have been developed, but, although laboratory studies may show a benefit, none significantly improved clinical outcomes. The use of poorly designed testing and lack consideration the impact VBNC populations be responsible.

Bacterial cells are known to change the fatty acid (FA) composition of the phospholipids as a phenotypic response to environmental conditions and to the presence of toxic compounds such as antibiotics. In the present study, Staphylococcus aureus cells collected during the exponential growth phase were challenged with 50 and 100 mg/L of vancomycin and teicoplanin, which are concentrations high e...

Despite the development of on-farm anaerobic digestion as a process for making profitable use of animal by-products, factors leading to the inactivation of pathogenic bacteria during storage of digestates remain poorly described. Here, a microcosm approach was used to evaluate the persistence of three pathogenic bacteria (Salmonella enterica Derby, Campylobacter coli and Listeria monocytogenes)...

The mode by which Helicobacter pylori, the causative agent of most gastric ulcers, is transmitted remains undetermined. Epidemiological evidence suggests these organisms are waterborne; however, H. pylori has rarely been grown from potential water sources. This may be due to the ability of this organism to rapidly enter the viable but nonculturable (VBNC) state. Our investigation examines the e...