this study was conducted to detect the common bacteria implicated in bovine mastitis in bulk tank milk by polymerase chain reaction (pcr). forty-four milk samples from bulk tank milk were obtained and submitted to our laboratory. to detect staphylococcus aureus, streptococcus agalactiae, streptococcus dysgalactiae, escherichia coli, streptococcus uberis, and streptococcus parauberis, two sets o...

A heterologous ELISA for measurement of osteocalcin (bone Gla-protein) is described, involving biotinylated bovine osteocalcin and polyclonal antibodies. The log-linear range was 2.3-37.5 micrograms/L. Between-run (total) and within-run CVs (n = 10) were 5.7-6.4 and 5.9-6.1%, respectively; analytical recoveries ranged from 92% to 108%. Comparison of our method (x) with an RIA (y) yielded y = 1....

Benefits of Bovine Colostrum on Fecal Quality in Recently Weaned Puppies Catriona J. Giffard,* Mitsuro M. Seino,y Peter J. Markwell,** and Roger M. Bektash* *Research and Development, Masterfoods Australia New Zealand, Wodonga, Vic 3690, Australia, yMaster Foods Japan Research and Development, Asashi Yoshikawa-shi, Saitama-ken, Japan, and **WALTHAM Centre for Pet Nutrition, Waltham-on-the-Wolds...

Genomic DNA extracted from bovine mummified tissue is valuable material for detection of some genes that may contribute to fetal abnormalities. In this study bovine genomic DNA was extracted from the hardened tissue samples of ten bovine mummified fetuses. The amount of genomic DNA extracted from 2 g of the mummified tissues by the phenol/chloroform-ethanol method was low (less than 4 microg/ml...

This paper demonstrates that a group of proteins, representing a major secretory component of cattle conceptuses, is immunologically related to ovine trophoblast protein-1 (oTP-1), a principal product of culture Day 13 to 21 sheep conceptuses. Conceptuses from cows (Day 17-18) and ewes (Day 16-17) were cultured for 24 h in the presence of L-[3H]leucine. By using a rabbit antiserum to oTP-1 and ...

The PRAME gene family belongs to the group of cancer/testis genes whose expression is restricted primarily to the testis and a variety of cancers. The expansion of this gene family as a result of gene duplication has been observed in primates and rodents. We analyzed the PRAME gene family in Eutheria and discovered a novel Y-linked PRAME gene family in bovine, PRAMEY, which underwent amplificat...

background : the aim of this study was to investigate the association between the two most commonly used generic and disease specific health-related quality of life (hrqol) measures in patients with chronic lung disease due to sm: medical outcomes study short form 36-item (sf-36) and st george's respiratory questionnaire (sgrq). methods : this is a secondary analysis of iranian chemical warfare...

background and objectives: a new real- time pcr protocol for the detection of trichomonas vaginalis in pooled urine samples has been optimized and validated. materials and methods: the amplification protocol, targeting a 2kb repeated gene in the t. vaginalis genome, was optimized by varying pcr parameters. as a reference method, a real-time pcr protocol targeting the beta-tubulin gene (y. versl...