نتایج جستجو برای: blocking elisa

تعداد نتایج: 95866  

2012
Kai Samelin Henrich Christopher Pöhls Arne Bilzhause Joachim Posegga Hermann de Meer

In this paper, we present a provably secure redactable signature scheme allowing to independently redact structure and content. We identify the problems when structure is not separated from content, resulting in an attack on the scheme proposed at VLDB ’08 by Kundu and Bertino. The attack allows for changing the semantic meaning of a given tree. We introduce a rigid security model, including co...

Journal: :Clinical chemistry 1994
W Y Craig S E Poulin C P Nelson R F Ritchie

We describe an ELISA for serum IgG antibodies against malondialdehyde-modified low-density lipoprotein (mLDL). Optimal antigen concentration, serum dilution, and dilution of enzyme-conjugated second antibody were 25 mg/L, 1:250, and 1:5000, respectively, when 5 g/L human serum albumin was used for blocking. When data were expressed as mLDL/LDL (the ratio of IgG binding to mLDL vs LDL), within-r...

Objective(s): New generation of allergy vaccines is capable of promoting the development of protective IgG and blocking the functionality of allergen-specific IgE. We incorporated universal and powerful T-cell epitopes from tetanus and diphtheria toxoids (TD epitope) into recombinant Che a 2, the well-known allergic profilin of Chenopodium album, to determine its immun...

2013
RAMESH JOSHI

Mastitis is known to be an economically important disease hampering desired progress in the dairy industry. Mastitis caused by Streptococci is of the subclinical type.Early detection of mastitis is of paramount importance to avoid permanent damage to the udder and loss of milk.The presently detection methods of Electrical Conductivity test (EC) and Somatic Cell Count (SCC) for subclinical bovin...

Journal: :Medycyna Weterynaryjna 2023

Foot-and-mouth disease (FMD) and bluetongue (BT) are two of the most devastating viral diseases affecting domestic wild ruminants. They cause significant economic losses, vaccination is important method for combating both FMD BT. Simultaneous vaccinations crucial animal welfare as well labor cost savings. No reports were found on simultaneous administration BT vaccines to sheep. This study eval...

Journal: :Clinical and vaccine immunology : CVI 2009
M A Loroño-Pino J A Farfan-Ale B J Blitvich J L Beebe R G Jarman B J Beaty

An epitope-blocking enzyme-linked immunosorbent assay (b-ELISA) was evaluated for the diagnosis of West Nile virus (WNV) infections in humans. Sera from patients diagnosed with WNV infections from an outbreak in 2003 in Colorado and from patients diagnosed with dengue virus infections from Mexico and Thailand were tested with the b-ELISA. The b-ELISAs were performed using the WNV-specific monoc...

Journal: :Experimental parasitology 1995
W Roeffen P J Beckers K Teelen T Lensen R W Sauerwein J H Meuwissen W Eling

The activity of monoclonal antibodies (mAbs) that specifically recognize the Plasmodium falciparum sexual stage-specific protein Pfs230 was analyzed. All mAbs reacted with the surface of extracellular sexual forms of the parasite in a suspension immunofluorescence antibody reaction and precipitated the Pfs230 protein from an NP-40 extract of surface radioiodinated macrogametes/zygotes. Only mAb...

Journal: :Investigative ophthalmology & visual science 2010
Heather L Chandler Kathleen S Reuter Loraine T Sinnott Jason J Nichols

PURPOSE To determine whether class I ultraviolet (UV) light-blocking contact lenses prevent UV-induced pathologic changes in a rabbit model. METHODS Twelve rabbits were assigned to 1 of 3 treatment groups (n = 4), as follows: senofilcon A (class I UV blocking) contact lenses; lotrafilcon A contact lenses (no reported UV blocking); no contact lens. The contralateral eye was patched without a c...

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