نتایج جستجو برای: chromosome manipulation
تعداد نتایج: 181117 فیلتر نتایج به سال:
Here, we present a novel method for the directed genetic manipulation of the Bacillus subtilis chromosome free of any selection marker. Our new approach employed the Escherichia coli toxin gene mazF as a counter-selectable marker. The mazF gene was placed under the control of an isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible expression system and associated with a spectomycin-resistanc...
Y chromosomes control essential male functions in many species, including sex determination and fertility. However, because of obstacles posed by repeat-rich heterochromatin, knowledge of Y chromosome sequences is limited to a handful of model organisms, constraining our understanding of Y biology across the tree of life. Here, we leverage long single-molecule sequencing to determine the conten...
Cotton is an economically important crop that needs more extensive genetic characterization. Highly effective methods for consistent cell cycle manipulation are needed to efficiently produce high quality cytological preparations of chromosomes, and to improve methods of chromosome doubling, both of which impact cotton genomics and breeding. This manuscript reports a procedure for cell cycle syn...
Human genes have an important role in physical features, behavior and mental diseases. Accordingly, a human being on whom the genetic manipulation has been done would be different from others. Can s/he bring a civil or criminal claim against physicians? Does the genetic manipulation make any specialized right for likely victim? To analyze such physicians' responsibility, Firstly, related descri...
Background: Frozen shoulder is a debilitating disease characterized by pain and progressive loss of motion in shoulder secondary to fibrotic inflammation of the joint capsule. The incidence is 2% in the general population, mostly affecting women 40 to 70 years of age. Therapeutic measures include physiotherapy, shoulder manipulation, corticosteroid injection and arthroscopic release. The purpos...
The present paper focuses on the questions of modern advertising science, structure of advertising and elements making actual manipulative influence from the addresser. Advertising encourages product sales, is an instrument of forming ethical standards, values, creating cultural values, standards and mode of behavior that is why the wide system of means for achieving aims of advertisers is need...
ABSTRACT Endometriosis is a complex disease, influenced by genetic factors. Genetic markers associated with endometriosis exist at chromosome 1p36.12 and lead to altered expression of the long intergenic non-coding RNA 339 (LINC00339), however, role LINC00339 in pathophysiology remains unknown. The aim this work was characterize patterns mRNA endometrium endometriotic lesions situ determine fun...
Different ploidy levels, diploid (2n=2x=24) to hexaploid (2n=6x=72) have been reported for Acorus calamus, a perennial medicinal plant. According to available information, there is a significant relationship between the ploidy level and essential oil compositions and medicinal properties of this important genetic resource. However, there is no information about the chromosome number of A. calam...
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