However, early efforts to incorporate AI into production schemes were limited by the functional lifespan of ejaculated spermatozoa. Therefore, widespread the use of AI Cryopreservation (CP) is the ability to store cells and maintain their integrity and viability at a sub-zero temperature until needed. Like some of the greatest discoveries, CP of semen was the result of a fortunate lab error. In...

Cryo banking of spermatozoa is an essential aspect of fertility preservation. With the advancements in cryobiology and better understanding of cryoprotectants and assisted reproduction, indications for semen banking are expanding. The exponential developments that have occurred over the years in the field of cryopreservation have proved that frozen sperm is as good as fresh sperm in fertilizing...

Background Cryopreservation procedure for poultry semen is not reliable due to structural and biochemical damages which can lead to reduction in sperm quality and fertility. Development of cryopreservation medium has crucial effects for successful recovery of sperm after freezing. The objective of this study was assessment of cryopreservation of rooster semen using hand-made cryopreservation me...

Introduction. Tendon grafts are widely demanded in reconstructive plastic surgery. Allogeneic tendons potentially have a number of advantages. However, the method long-term storage allogeneic has not been optimized to date. Aim. Selection an optimal cryoprotectant for human tendon allografts at ultra-low temperatures, which allows preserving native tissue structure. Material and methods. We stu...

This review presents some of the most noticeable aspects related with the oocyte cryopreservation procedures, emphasizing their evolution in the bovine, which points towards the critical points determining the reduced survival rates of female gametes to freezing and vitrification. Factors such as the maturation status, the cytoskeleton and membrane sensitivity, the role of the cumulus cells, th...

For the first time, pre-hibernation sizes of “storage” organs (liver and fat bodies), type, concentration total content reserve substances (glycogen or lipids) in these muscle tissue, their change during freezing freeze tolerant specimens moor frog (Rana arvalis) Siberian population were studied. In autumn, relative weight liver (hepatosomatic index) studied individuals reaches 10%, which is 2–...