The first identification of a sterol in Chlorella was by Klosty and Bergmann (5) in 1952 when they isolated ergosterol from Chlorella pyrenoidosa. More recently, Otsuka (6) identified ergosterol and a A5 sterol in Chlorella ellipsoidea. Ergosterol was the principal component of the mixture. Recent work in this laboratory has been centered about the identification of sterols from other species o...

Vitamin D deficiency has been linked to various diseases, but could be rectified via fortified food stuffs or supplementation. In this study 39 different hydrophobic deep eutectic solvents were evaluated for green extraction of ergosterol from mushroom. Extraction parameters (e.g. time, solvent volume) optimized using response surface methodology (RSM) and a maximum yield 6995.00 µg ergosterol/...

Amphotericin B (AmB)-resistant Leishmania donovani promastigotes were selected by increasing drug pressure, and their biological features were compared with those of the wild-type parent strain. The 50% inhibitory concentration for resistant cells was 20 times higher than that for the wild-type. Resistance was stable after more than 40 passages in drug-free medium, and resistant promastigotes w...

We have studied the effect of ergosterol, an important component of fungal plasma membranes, on the physical properties of dipalmitoylphosphatidylcholine (DPPC) multibilayers using deuterium nuclear magnetic resonance ((2)H NMR) and differential scanning calorimetry (DSC). For the (2)H NMR experiments the sn-1 chain of DPPC was perdeuterated and NMR spectra were taken as a function of temperatu...

Genome-wide expression analysis of an industrial strain of Saccharomyces cerevisiae during the initial stages of an industrial lager fermentation identified a strong response from genes involved in the biosynthesis of ergosterol and oxidative stress protection. The induction of the ERG genes was confirmed by Northern analysis and was found to be complemented by a rapid accumulation of ergostero...

In a previous publication (1) it was shown that the feeding of cod liver oil to dogs for a period of 2 weeks before the removal of the thyroid and parathyroid glands greatly influenced the results of such an operation. The calcium of the blood serum fell as usual after parathyroidectomy, but the animals either did not develop tetany or the attacks were slight. The length of life of the treated ...

In the previous paper it was shown that highly purified spinal cord cholesterol is still activatable to antirachitic activity when irradiated in the solid form by a Cooper Hewitt mercury vapor quartz lamp. Although the activatability is decreased by the purification methods employed, nevertheless these purified products can again be rendered at least 25 times more activatable if heated under th...

In Candida albicans, lipid rafts (also called detergent-resistant membranes, DRMs) are involved in many cellular processes and contain many important proteins. In our previous study, we demonstrated that Rta2p was required for calcineurin-mediated azole resistance and sphingoid long-chain base release in C. albicans. Here, we found that Rta2p was co-localized with raft-constituted ergosterol on...

Sterols are widely distributed in nature. It has been suggested that they be classified into three groups according to their occurrence : (1) zoosterols, the animal sterols (cholesterol, coprosterol) ; (2) phytosterols, the plant or vegetable sterols (sitosterol, stigmasterol) ; and (3) mycosterols, the sterols found in fungi (ergosterol, fungisterol). Although ergosterol has been detected in s...