simula in vivo and to of thi operat muscl from k and S video locom positio during muscl injection of a fixative solution, usually formaldehyde or glutaraldehyde. Muscle fibres are then dissected out and sarcomere length determined microscopically or by light diffraction. James et al. (1995) examined the properties of mouse fast twitch (extensor digitorum longus, EDL) and slow twitch (soleus) mu...

Ultra-structural analysis of human post-mortem brain tissue is important for investigations into the pathomechanism of neuropsychiatric disorders, especially those lacking alternative models of studying human-specific morphological features. For example, Von Economo Neurons (VENs) mainly located in the anterior cingulate cortex and in the anterior part of the insula, which seem to play a role i...

For localization of pyroantimonate-precipitable cations, mouse testes were fixed with a saturated aqueous solution of potassium pyroantimonate (pH about 9 .2, without addition of any conventional fixative), hardened with formaldehyde, and postosmicated . A good preservation of the cell membranes and over-all cell morphology is obtained as well as a consistent and reproducible localization of th...

Gangliosides, cell surface glycosphingolipids, are implicated in diverse biologic functions potentially important for tumor growth. Because expression of the GD3 ganglioside may have an impact on the melanoma malignancy, and therefore on the patient prognosis, we evaluated the feasibility of a retrospective immunohistochemical study of GD3 in paraffin embedded biopsies of primary melanomas. Imm...

Serial dilution and agar overlay techniques were used to compare the cytotoxicity of formocresol, 19% formaldehyde, 35% cresol, and 2.5% glutaraldehyde to human pulp fibroblasts. The maximum nontoxic concentration of each agent was determined to allow quantitative comparisons both of the agents tested and of the techniques used. Formaldehyde was found to be the major component of formocresol wh...

INTRODUCTION High-resolution MRI characterizations of diffusion anisotropy in formaldehyde-fixed autopsy tissue samples may better characterize cytoarchitecture in normal and injured human nervous tissue than in vivo samples. Unfortunately, logistics and patient family needs dictate that autopsy tissues are usually obtained following a 4+ hour postmortem interval (PMI). The stigmata of PMI can ...

A fluorescein isothiocyanate (FITC) technique and one based on peroxidase-antiperoxidase (PAP) were used to study the distribution of immunoglobulin (Ig) in cryostat and paraffin sections of human tonsil. Trypsin and other proteolytic enzymes were used to 'unmask' the antigen in paraffin sections. The effects of processing, and particularly of fixation, on the immunohistochemical response of ti...

For localization of pyroantimonate-precipitable cations, mouse testes were fixed with a saturated aqueous solution of potassium pyroantimonate (pH about 9.2, without addition of any conventional fixative), hardened with formaldehyde, and postosmicated. A good preservation of the cell membranes and over-all cell morphology is obtained as well as a consistent and reproducible localization of the ...

The permeability of the alveolar-capillary membrane of newborn and adult mice to horseradish peroxidase (HRP) and catalase was studied by means of ultrastructural cytochemistry, and the permeability to ferritin was studied by electron microscopy. The influence of varying volumes of intravenously injected fluid on the rate of leakage of the tracers from pulmonary capillaries was examined. The tr...