Background: The use of C-type natriuretic peptide (CNP) combined with cysteamine during pre-in vitro maturation (IVM) can help establish an effective pre-IVM system. Aims: This study was designed to investigate the effects of CNP combined with cysteamine on meiotic maturation and developmental competence of bovine oocytes in vitro. Met...

the aim of this study was to investigate the in vitro developmental competence of isolated pre-antral follicles derived from vitrified ovaries in the presence of coenzyme q 10 (coq 10 ) . mice pre-antral follicles derived from fresh and vitrified-warmed ovarian tissues were in vitro cultured individually in α-mem medium supplemented with or without coq 10 , followed by adding human chorionic go...

objective: crocin is an active ingredient of saffron (crocus sativus l.) and its antioxidant properties have been previously investigated. this carotenoid scavenges free radicals and stimulates glutathione (gsh) synthesis; consequently, it may protect cells against oxidative stress. the aim of this research is to protect oocytes from oxidative stress by the addition of a natural source antioxid...

Background: Polycystic ovary syndrome (PCOS) is a multifactorial, heterogeneous, complex genetic endocrine and metabolic disorder, diagnostically characterized by chronic anovulation, polycystic ovaries and biochemical and clinical manifestations of hyperandrogenism. In patients with PCOS lower apoptotic rates found in granulosa cells compared to women with regular ovulatory cycles and this lea...

Background: The study was carried out to investigate the relationship between the development potential and the diameter of ovine oocytes. Materials and Methods: Ovaries collected from a local abattoir were transported to the laboratory within 1-3 h of slaughter. The oocytes from follicles (2-6 mm in diameter) were recovered by aspiration and stored in a preincubated (at 38.6 C, 5% CO2 and maxi...

the aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (cocs). bovine ovaries were collected from the local slaughterhousein a container with 30-37°c physiologic saline and supplemented with antibiotics. the grade a aspiratedcocs were assigned to 6 experimental groups; the 1st group was control (n=100) in whi...

Background: Different culture media are used for in vitro maturation of ovine oocyte. The Weymouth medium are used for in vitro maturation of rat and mice oocytes with very low levels of supplementation (Just FBS, while to acquire the good results in TCM hormone, and serum supplementation and in some cases growth factor are necessary to gain the optimized results. The aim of the present study w...

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...