This research studies the application of essential oil nanoemulsion as herbal medicine instead of using antibiotics and chemicals. Thyme, shirazi thyme and rosemary essential oils were selected as herbal drugs. Essential oil nanoemulsions with Tween 80 and/or Sodium dodecyl sulfate (SDS) surfactants were prepared and investigated. Physicochemical characterizations such as hydrodynamic di...

We have developed a simple in vitro translation method to analyze compounds that inhibit the rhinovirus 3C protease in peptide substrate assays but demonstrate no antiviral activity. This complementary assay, which provides both qualitative and quantitative results, detects the inhibition of the 3CD protease in the native polyprotein form.

The influence of an upstream open reading frame (ORF) in the 5'-untranslated region (UTR) of the mRNA on corticotropin-releasing hormone receptor type 1 (CRHR1) translation was studied in constructs containing the 5'-UTR of CRHR1, with or without an ATG-to-ATA mutation in the upstream ORF, and the main ORF of luciferase or CRHR1. Upstream mutation in luciferase constructs increased luciferase a...

Long noncoding RNAs (lncRNAs) are important regulators of transcription; however, their involvement in protein translation is not well known. Here we explored whether the lncRNA GAS5 is associated with translation initiation machinery and regulates translation. GAS5 was enriched with eukaryotic translation initiation factor-4E (eIF4E) in an RNA-immunoprecipitation assay using lymphoma cell line...

BENCHMARKS 9q and 20, where, respectively, chromo-somal loss and gain is frequently detected in SCA III ovarian tumors (7,8). After selecting the genes on these respective chromosomes, hierarchical clustering is performed. Sorting the genes on chromosomal order reveals particular chromosomal regions with under-or overexpressed genes in subsets of SCA III tumors. Possible retention or amplificat...