Commercial solid-phase extraction columns packed with a stationary phase with bonded benzenesulfonic acid groups are readily converted to the silver ion form and can then be used for silver ion chromatography of lipids. To illustrate the utility of such procedures, methyl ester derivatives of fatty acids with zero to six double bonds were separated from each other by a simple stepwise elution s...

of amino alcohols using Z-L-Glu-L-Pro or Z-L-Glu-D-Pro as chiral counter ions and Hypercarb as the solid phase. Chirality 9: 650}655. Knox JH and Jurand J (1982) Separation of optical isomers by zwitterion pair chromatography. Journal of Chromatography 234: 222}224. Knox JH and Ross P (1997) Carbon-based packing materials for liquid chromatography. Advances in Chromatography, 74}162. Petterson ...

a blocker of postsynaptic acetylcholine receptors was isolated from venom of the eastern green mamba dendroaspis angusticeps, by gel filtration, ion-exchange and reverse phase high performance liquid chromatography. the isolated component blocks neuromuscular transmission and responses to exogenously applied acetylcholine in isolated chick biventer cervices preparations. these results suggest t...

Size exclusion chromatography (SEC) has been widely applied in protein separation based on their molecular sizes. Here we present a peptide mixture from 1kD to 6 kD separation on size exclusion chromatography column Zenix TM -80. The effect of different mobile phases on the peptides’ separation performances was also explored. In the separation of insulin, glucagon, angiotensin I and bradykinin,...

■ CONTENTS Principles of Single Molecule Spectroscopy and Instrumental Techniques 84 General Considerations and Sample Requirements 84 Confocal Microscopy 85 Total Internal Reflectance Fluorescence Wide Field Imaging and Analysis 86 Chromatography Methods Studied by Single Molecule Spectroscopy 87 Reverse Phase Liquid Chromatography 87 Silica Surfaces: Normal Phase Chromatography and Capillary ...

Background and objectives: According to recent studies, homotaurine may be an Alzheimer's active ingredient. The goal of this project was to extract and detect homotaurine in algae collected from Persian Gulf shores. Methods: Extraction was done with etanol 80% for 30 min in ambient temperature. The HPLC elution solvent required a mixture of two solvents. Solve...

Effects of temperature and percentage of organic modifier were studied on retention and selectivity in RP-HPLC using solvation parameter model. The system constants were determined by multiple linear regression analysis from experimental values in the retention factor for a group of different solutes with known descriptors by computer using the program SPSS/PC. The experimental results showed t...