Background: Plasma membrane of the spermatozoa interacts with and is altered by abundant seminal PDC-109 present in their immediate milieu in vivo with damaging effect in a time- and concentration- dependent manner. Therefore we hypothesized that sequestration of detrimental protein from ejaculates would be beneficial for cryopreservation of sperm cells. To this aim we evaluated the effect of s...

The present study was aimed to observe the postthaw quality of Nili-Ravi buffalo bull semen cryopreserved during heat stress (hot summer months) and normal climatic conditions (fall, winter, spring months). For this purpose, four (n = 4) adult regular donor bulls were selected collect cryopreserve their throughout period an entire year. cryopreservation done following standard freezing protocol...

Low-Density Lipoprotein (LDL) extracted from egg yolk has recently known can eliminate the adverse effect associated in use of fresh yolk. The role LDL liquid preservation at 4°C ram sperm not been explored. This research evaluates effects substituting with 4 °C on 5 days. objective this was to assess for as an extender 4°C, well spermatozoa motility, viability, morphology, plasma membrane, and...

In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (...

the effect of different levels of osmolality and ph of lactose extender on the viability of bactrian camelspermatozoa was investigated. semen was collected from 10 bactrian camel bulls using modified bovineartificial vagina. in experiment i, the extenders consisted of 9, 10, 11, 12 and 13% lactose with respectiveosmolalities of 290, 333, 350, 376 and 419 mosm/kg and the ph of 6.9. in experiment...

experiment was conducted to evaluate the effect of l-glutamine on coated sperm freezing by using four rams. semen was collected by artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. samples were pooled and centrifuged. after removing supernatant and diluting pellet, samples were split into 5 equal part and added 0, 40, 80, 120 and 160 mm l-glutamine. samples were froze...

The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...

brown spot disease caused by alternaria alternata (fr.: fr.) keissl is a serious problem forproduction of tangerines and tangerine hybrids in iran. the tangerine pathotype causes brownspot disease on young leaves and immature fruits of limited varieties of mandarins and tangerines(citrus. reticulata blanco). specificity in the interaction between tangerine and the pathogen isdetermined by a hos...