نتایج جستجو برای: rapid detection
تعداد نتایج: 837934 فیلتر نتایج به سال:
Abstract Background and objectives: The aim of this study was the detection of S.pneumoniae infection by rapid urinary test and blood culture in children with pneumonia in comparison with healthy children. Material and Methods: This case control study was carried out in pediatric ward of Rasoul Akram hospital in Tehran, Iran (2006 - 200٧).Fifty-four Community acquired pneumonia (CAP) and 50 hea...
Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...
Background: Somatic mutations in the hotspot region of the DNA-methyltransferase 3A (DNMT3A) gene were recurrently identified in acute myeloid leukemia (AML). It is believed that DNMT3A mutations confer an adverse prognosis for AML patients. These lines of evidence support the need for a rapid and cost-efficient method for the detection of these mutations. The present study aimed to establish h...
Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...
Background and purpose: Molecular detection of antibiotic resistance in clinical strains of M.tuberculosis is of great importance. In this study, we developed a method for rapid detection of mutations resistant to the rifabutin antibiotic resistant gene. Material and methods: In this study 40 clinical isolates of M.tuberculosis including 12 resistant and 28 susceptible isolates to rifabutin we...
Avian influenza virus (AIV) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. Methods: A multiplex Reverse Transcriptase PCR (RT-PCR) was optimized for the detection of influenza A virus and the H5 and H9 subtypes. The influenza type A specific primers were directed to t...
objective: toxoplasmosis may cause significant damage to the developing fetus and is a life-threatening opportunistic infection in immunocompromised persons. molecular methods are known to be more sensitive and more specific than serological assays for diagnosis of toxoplasmosis. application of quantitative pcr has evolved sensitive, specific, and rapid method for the detection of rh strain of ...
Introduction: Detection of enteroviruses and mumps virus nucleic acid in cerebrospinal spinal fluid (CSF) specimens has been demonstrated to improve the management of patients with aseptic meningitis. Then rapid and sensitive diagnostic techniques are desirable. Materials & Methods: Multiplex RT-PCR has the potential to detection both of viruses. This assay is so rapid and sensitive. At fir...
Background : In many patients with chest pain, the correct diagnosis of acute myocardial infarction(AMI) is dependent mainly on the measurement of cardiac markers such as cardiac creatin kinase isoenzyme (CK-MB) and cardiac troponin I (cTnI) ,because the electrocardiogram is often non-diagnostic. The measurement of cardiac markers are superior for the detection of minor myocardial injury and mo...
marek’s disease (md) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. the present study was an attempt to use polymerase chain reaction (pcr) tooptimize a rapid and reliable assay for detection of mdv genome. detection of serotype 1 of mdv (mdv-1)was confirmed by presence of a 200 bp dna fragment as a pcr product. differentiation of mdv-1 ...
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