rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. in this study we investigated the effects of supplementation of cysteamine on the rate of in vitro maturation of oocytes in two different media. germinal vesicle oocytes were collected from mouse ovary and cultured in two media (tcm199 and meme) with 0, 50, 100, 200, 500 µm/ml cysteamine. number...

in vitro maturation (ivm) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. this review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. the review is based on literature reports and the author’s experience. in ivm cycles,...

developing a culture system for preantral follicles has important biotechnological implications due to the potential to produce a large number of oocytes for embryo production and transfer. to accomplish this goal, the present study was aimed to culture preantral follicles in the presence of different media, sera and fsh concentrations. six-week-old preantral follicles (95 ± 5 µm) were cultured...

background: human chorionic gonadotropin (hcg) has been used as a replacement for the mid-cycle luteinizing hormone (lh) surge for several years. the recent arrival of recombinant dna technology has made recombinant hcg (rhcg) accessible. objective: to assess efficacy of rhcg compared to urinary hcg (uhcg) for triggering of ovulation and induction of final oocyte maturation in assisted reproduc...

objective: currently, there is no agreement on the optimal urinary derived human chorionic gonadotropin (u-hcg) dose requirement for initiating final oocyte maturation prior to oocyte collection in in vitro fertilization (ivf), but doses that range from 2500- 15000 iu have been used. we intended to determine whether low dose u-hcg was effective for oocyte maturation in ivf/intracytoplasmic sper...

the use of ovarian stimulation for infertility treatment is associated with side effects of ovarian hyperstimulation syndrome (ohss) and potential cancer risk. this is also true in high risk women such as those polycystic with ovary (pco) and polycystic ovarian syndrome (pcos). in vitro maturation (ivm) of oocytes was primarily developed to make ivf safe for women with pco and at high risk of o...

Background: Oocyte maturation is usually defined as the period of progression from the first to the second meiotic arrest and involves coordinated nuclear and cytoplasmic modification. Ca2+ signals are known as important regulators of oocyte maturation. Extracellular Ca2+ is important for first polar body formation, normal meiosis kinetics, and for preserving the chromosome and spindle configur...

background & objectives : different energy substrates and nutrients can greatly influence oocyte nuclear and cytoplasmic maturation. effect of glucose on in-vitro oocyte maturation and subsequent early embryonic development is controversial. the objective of the present study was to verify the effect of various concentrations of glucose on cumulus-oocyte complexes maturation, fertilization and ...