the goal of this study was the evaluation of semen extender filtration on post-thawed buck sperm quality. a total 16 ejaculates were collected from four buck (4 ejaculates from each buck) twice a week. after semen collection and primary evaluation, collected semen samples were pooled and divided into two equal parts. each part was diluted with one of the following extenders: 1) non-filtered lec...

the aim of the present research was to study the effect of different concentrations of rosemary (rosmarinus officinalis l.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/l added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 ross strain rooster were used. semen samples were collected, 2 times a week. after adding the samples ...

Egg yolk low-density lipoproteins (LDL) and soybean lecithin were evaluated as replacements for egg yolk in extenders used for the cryopreservation of brown-bear spermatozoa. The motility, viability and acrosomal status of post-thawed spermatozoa were analysed, and an egg-yolk extender was used as a control. The total antioxidant capacity of these extenders was tested. Soybean lecithin showed a...

The objective of this study was to specify the effects of centrifugation and different surface of soybean lecithin-based extender on post-thaw Sperm quality of Ghezel ram semen. Semen samples were gathered from 5 mature Ghezel ram two/week for 3 weeks. After initial evaluation, the approved semen samples were pooled together and split into equal parts in Falcon tubes. Three samples were combine...

Adding antioxidants to semen extenders could reduce post-thaw sperm damages. Glutathione is an important intracellular antioxidant that has free radicals scavenging properties and multiple effects on DNA and protein synthesis. In the present study, effects different levels of reduced glutathione (GSH) containing 0 (control), 1.0, 2.5, or 5.0 mM in a nanomicelle-based extender on post-thaw quali...

the aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. in this experiment, different concentrations of glutathione [0 (lg-0), 0.5 (lg-0.5), 1 (lg-1), 1.5 (lg-1.5), and 2 (lg-2) mm] were added in a soybean lecithin-based extender. a total of 16 ejaculates from four bucks were collected and pooled. each pooled sample was divided into five...

the effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°c was evaluated. semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body weights of 64 kg. semen samples were pooled and divided into 4 parts. each part was diluted with...

The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five...

Background: Trehalose, a non-permeable disaccharide, has a protective role related both to osmotic effect and specific interactions with membrane phospholipids, rendering hypertonic media, causing cellular osmotic dehydration before freezing, and then decreasing the amount of cell injury by ice crystallization. Within this study, an attempt was made to evaluation of two levels of 50 and 100 mM ...